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λb221poriCasnA的分离与特性鉴定，λb221poriCasnA是一种携带大肠杆菌染色体复制起点的噬菌斑形成特异性转导噬菌体。

Isolation and characterization of lambda b221poriCasnA, a plaque-forming specialized transducing phage carrying the origin of replication of the Escherichia coli chromosome.

作者信息

Soll L

出版信息

Mol Gen Genet. 1980;178(2):381-9. doi: 10.1007/BF00270488.

DOI:10.1007/BF00270488
PMID:6446649
Abstract

A specialized transducing phage lambda b221poriCasnA has been isolated carrying oriC the origin of chromosomal replication of Escherichia coli. All phage genes required for lytic growth are retained, thus the phage is capable of lytic growth. The presence of the oriC locus confers upon infecting phage DNA the ability to replicate as a plasmid using only host DNA replication functions. The presence of both oriC and ansA markers has allowed the development of a plaque assay for origin function which can be used to identify mutants at these loci. Comparison of restriction endonuclease cleavage sites present on lambda b221proiCasnA DNA to those on its parent, lambda b221 rex::Tn10 suggests the steps involved in the formation of the transducing phage.

摘要

一种携带大肠杆菌染色体复制起点oriC的特异性转导噬菌体λb221poriCasnA已被分离出来。保留了裂解生长所需的所有噬菌体基因，因此该噬菌体能够进行裂解生长。oriC位点的存在赋予感染的噬菌体DNA仅利用宿主DNA复制功能作为质粒进行复制的能力。oriC和ansA标记的存在使得能够开发一种用于起点功能的噬菌斑测定法，该方法可用于鉴定这些位点的突变体。将λb221proiCasnA DNA上存在的限制性内切酶切割位点与其亲本λb221 rex::Tn10上的切割位点进行比较，揭示了转导噬菌体形成过程中涉及的步骤。

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Isolation and characterization of lambda b221poriCasnA, a plaque-forming specialized transducing phage carrying the origin of replication of the Escherichia coli chromosome.λb221poriCasnA的分离与特性鉴定，λb221poriCasnA是一种携带大肠杆菌染色体复制起点的噬菌斑形成特异性转导噬菌体。
Mol Gen Genet. 1980;178(2):381-9. doi: 10.1007/BF00270488.
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引用本文的文献

1
Pseudovirulent mutants of lambda b221poriCasnA resulting from mutations in or near oriC, the E. coli origin of DNA replication.由大肠杆菌DNA复制起点oriC及其附近的突变产生的λb221poriCasnA假毒力突变体。
Mol Gen Genet. 1980;178(2):391-6. doi: 10.1007/BF00270489.
2
UV irradiation inhibits initiation of DNA replication from oriC in Escherichia coli.紫外线照射会抑制大肠杆菌中oriC处DNA复制的起始。
Mol Gen Genet. 1989 Apr;216(2-3):446-54. doi: 10.1007/BF00334389.

本文引用的文献

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[Genetic study of a temperate bacteriophage of Escherichia coli. l. The genetic system of the bacteriophage].[大肠杆菌一种温和噬菌体的遗传学研究。I. 噬菌体的遗传系统]
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Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.阿拉伯糖操纵子的定向转座：一种用于分离针对任何大肠杆菌基因的特异性转导噬菌体的技术。
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Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.大肠杆菌中的超螺旋环状DNA-蛋白质复合物：纯化及诱导转化为开放环状DNA形式
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66. doi: 10.1073/pnas.62.4.1159.
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Strains of phage lambda in current use.目前使用的λ噬菌体菌株。
Virology. 1969 Jun;38(2):349-51. doi: 10.1016/0042-6822(69)90378-x.