Department of Epidemiology and Medical Statistics, Guangdong Medical University, Dongguan, Guangdong 523808, China.
Huizhou Central People's Hospital, Department of Endocrinology, Huizhou, Guangdong 516008, China.
Biomed Res Int. 2020 Nov 13;2020:3816056. doi: 10.1155/2020/3816056. eCollection 2020.
Noncoding RNAs (ncRNAs) play an important role in the occurrence and development of type 2 diabetes mellitus (T2DM). This paper summarized the current evidences of the involvement microRNAs, long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs) in the differential expressions and their interaction with each other in T2DM.
The differentially expressed miRNAs, lncRNAs, and circRNAs in the blood circulation (plasma, serum, whole blood, and peripheral blood mononuclear cells) of patients with T2DM were found in PubMed, GCBI, and other databases. The interactions between ncRNAs were predicted based on the MiRWalk and the DIANA Tools databases. The indirect and direct target genes of lncRNAs and circRNAs were predicted based on the starBase V2.0, DIANA Tools, and LncRNA-Target databases. Then, GO and KEGG analysis on all miRNA, lncRNA, and circRNA target genes was performed using the mirPath and Cluster Profile software package in R language. The lncRNA-miRNA and circRNA-miRNA interaction diagram was constructed with Cytoscape. The aim of this investigation was to construct a mechanism diagram of lncRNA involved in the regulation of target genes on insulin signaling pathways and AGE-RAGE signaling pathways of diabetic complications.
A total of 317 RNAs, 283 miRNAs, and 20 lncRNAs and circRNAs were found in the circulation of T2DM. Dysregulated microRNAs and lncRNAs were found to be involved in signals related to metabolic disturbances, insulin signaling, and AGE-RAGE signaling in T2DM. In addition, lncRNAs participate in the regulation of key genes in the insulin signaling and AGE-RAGE signaling pathways through microRNAs, which leads to insulin resistance and diabetic vascular complications.
Noncoding RNAs participate in the occurrence and development of type 2 diabetes and lead to its vascular complications by regulating different signaling pathways.
非编码 RNA（ncRNA）在 2 型糖尿病（T2DM）的发生和发展中起重要作用。本文总结了 microRNA、长链非编码 RNA（lncRNA）和环状 RNA（circRNA）在 T2DM 中差异表达及其相互作用的研究现状。
在 PubMed、GCBI 等数据库中查找 T2DM 患者血液（血浆、血清、全血和外周血单个核细胞）中差异表达的 microRNA、lncRNA 和 circRNA。根据 MiRWalk 和 DIANA Tools 数据库预测 ncRNA 之间的相互作用。根据 starBase V2.0、DIANA Tools 和 LncRNA-Target 数据库预测 lncRNA 和 circRNA 的间接和直接靶基因。然后，使用 R 语言中的 mirPath 和 Cluster Profile 软件包对所有 miRNA、lncRNA 和 circRNA 靶基因进行 GO 和 KEGG 分析。使用 Cytoscape 构建 lncRNA 参与调节糖尿病并发症胰岛素信号通路和 AGE-RAGE 信号通路靶基因的机制图。
在 T2DM 患者的循环中发现了 317 种 RNA、283 种 microRNA、20 种 lncRNA 和 circRNA。失调的 microRNA 和 lncRNA 参与了 T2DM 中与代谢紊乱、胰岛素信号和 AGE-RAGE 信号相关的信号。此外，lncRNA 通过 microRNA 参与胰岛素信号和 AGE-RAGE 信号通路关键基因的调节，导致胰岛素抵抗和糖尿病血管并发症。
非编码 RNA 通过调节不同的信号通路参与 2 型糖尿病的发生发展，并导致其血管并发症。
