Department of Oral & Cranio-Maxillofacial Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.
Department of General Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.
J Nanobiotechnology. 2022 Mar 27;20(1):162. doi: 10.1186/s12951-022-01361-5.
The repair of tissue defects has attracted considerable attention and remained a substantial challenge. Calcium silicate (CaSiO, CS) bioceramics have attracted the interest of researchers due to their excellent biodegradability. Recent studies have demonstrated that nanoscale-modified bioactive materials with favorable biodegradability could promote bone tissue regeneration, providing an alternative approach for the repair of bone defects. However, the direct construction of biodegradable nanostructures in situ on CS bioceramics was still difficult.
In this study, flower-like nanostructures were flexibly prepared in situ on biodegradable CS bioceramics via hydrothermal treatment. The flower-like nanostructure surfaces exhibited better hydrophilicity and more significantly stimulated cell adhesion, alkaline phosphatase (ALP) activity, and osteogenic differentiation. Furthermore, the CS bioceramics with flower-like nanostructures effectively promoted bone regeneration and were gradually replaced with newly formed bone due to the favorable biodegradability of these CS bioceramics. Importantly, we revealed an osteogenesis-related mechanism by which the FAK/p38 signaling pathway could be involved in the regulation of bone mesenchymal stem cell (BMSC) osteogenesis by the flower-like nanostructure surfaces.
Flower-like nanostructure surfaces on CS bioceramics exerted a strong effect on promoting bone repair and regeneration, suggesting their excellent potential as bone implant candidates for improving bone regeneration.
组织缺损的修复引起了广泛关注，仍然是一个巨大的挑战。硅酸钙（CaSiO3，CS）生物陶瓷由于其优异的生物降解性而引起了研究人员的兴趣。最近的研究表明，具有良好生物降解性的纳米改性生物活性材料可以促进骨组织再生，为骨缺损的修复提供了一种替代方法。然而，在 CS 生物陶瓷上直接构建可生物降解的纳米结构仍然具有一定的难度。
本研究通过水热处理，在可生物降解的 CS 生物陶瓷上灵活地原位制备了花状纳米结构。花状纳米结构表面表现出更好的亲水性，更显著地刺激细胞黏附、碱性磷酸酶（ALP）活性和成骨分化。此外，由于 CS 生物陶瓷具有良好的生物降解性，具有花状纳米结构的 CS 生物陶瓷能够有效促进骨再生，并逐渐被新形成的骨所替代。重要的是，我们揭示了一个成骨相关的机制，即花状纳米结构表面通过 FAK/p38 信号通路参与调节骨髓间充质干细胞（BMSC）的成骨作用。
CS 生物陶瓷上的花状纳米结构表面对促进骨修复和再生具有很强的作用，表明其作为改善骨再生的骨植入物候选材料具有优异的潜力。
