Hartmann G, Högenauer G
Mol Gen Genet. 1978 May 31;161(3):231-6. doi: 10.1007/BF00330995.
With the aid of DNA-RNA hybridization experiments it was shown that an E. coli strain harbouring the resistance factor R1 contains small, stable RNA species which are capable of annealing with the DNA of this R-factor. At a higher hybridization temperature RNA molecules form the isogenic, sensitive strain could also hybridize to the R1-DNA. These RNA-species were again detected if the RNA was extracted from P. mirabilis carrying this plasmid. The fact that sulfur-labelled RNA also hybridized to the plasmid DNA seems to indicate that some of these molecules are tRNAs.
借助DNA-RNA杂交实验表明，携带抗性因子R1的大肠杆菌菌株含有小的、稳定的RNA种类，它们能够与该R因子的DNA退火。在较高的杂交温度下，来自同基因敏感菌株的RNA分子也能与R1-DNA杂交。如果从携带该质粒的奇异变形杆菌中提取RNA，也能再次检测到这些RNA种类。硫标记的RNA也能与质粒DNA杂交这一事实似乎表明，这些分子中的一些是转运RNA。
