大肠杆菌中的重复DNA：与小稳定RNA互补的多个序列

Repetitive DNA in Escherichia coli: multiple sequences complementary to small stable RNAs.

作者信息

Bailey S C, Apirion D

机构信息

Department of Microbiology and Immunology, Washington University, School of Medicine, St. Louis, Missouri 63110.

出版信息

Mol Gen Genet. 1979;172(3):339-43. doi: 10.1007/BF00271734.

DOI:10.1007/BF00271734

PMID:95791

Abstract

Radioactively labeled 4.5S, 6S, and 10S RNAs from Escherichia coli were hybridized to EcoRI fragments from the E. coli genome. Each of these molecules bound to more than one DNA fragment. Cot curve analysis of the kinetics of the annealing of these RNAs to denatured E. coli DNA suggests that the DNA corresponding to each of these molecules is reiterated in the genome. These experiments also suggest that these reiterated sequences are non adjacent.

摘要

来自大肠杆菌的放射性标记的4.5S、6S和10S RNA与大肠杆菌基因组的EcoRI片段进行杂交。这些分子中的每一个都与不止一个DNA片段结合。对这些RNA与变性大肠杆菌DNA退火动力学的Cot曲线分析表明，与这些分子中的每一个相对应的DNA在基因组中是重复的。这些实验还表明，这些重复序列是非相邻的。