Thomas L H, Howard C J, Stott E J, Parsons K R
Vet Pathol. 1986 Sep;23(5):571-8. doi: 10.1177/030098588602300505.
Mycoplasma bovis was inoculated alone or in combination with respiratory syncytial virus into the respiratory tracts of 12 gnotobiotic calves. Clinical signs ranged from transient pyrexia to protracted fever accompanied by severe lower respiratory signs and in one case, arthritis. Pulmonary lesions included foci of coagulative necrosis surrounded by mononuclear cells and suppurative bronchiolitis with varying degrees of lympho-reticular hyperplasia. No enhancement of lesions occurred in the combined infections of M. bovis and respiratory syncytial virus. M. bovis was identified by immunoperoxidase labelling in lesions of necrosis, especially at interfaces between the lesion and mononuclear cells and in bronchiolar exudates. Organisms were also located in necrotic lesions of joint capsules, in tonsillar crypts, and in liver.
将牛支原体单独或与呼吸道合胞病毒联合接种到12头无菌犊牛的呼吸道中。临床症状从短暂发热到持续发热，伴有严重的下呼吸道症状，其中1例还伴有关节炎。肺部病变包括凝固性坏死灶，周围有单核细胞，以及化脓性细支气管炎，并伴有不同程度的淋巴网状组织增生。牛支原体和呼吸道合胞病毒联合感染时，病变未加重。通过免疫过氧化物酶标记在坏死病变中，特别是在病变与单核细胞之间的界面以及细支气管渗出物中鉴定出牛支原体。病原体也存在于关节囊的坏死病变、扁桃体隐窝和肝脏中。
