Kellenberger-Gujer G, Podhajska A J, Caro L
Mol Gen Genet. 1978 Jun 1;162(1):9-16. doi: 10.1007/BF00333845.
A heat resistant mutant of E. coli dnaAts46 was isolated, which grows normally only at temperatures above 39 degrees. After a temperature shift from 42 degrees to 32 degrees the mutant overproduces DNA relative to protein. This is due to overinitiation of rounds of chromosome replication at low temperature, as indicated by hybridization and other experiments. The mutation is cotransduced by PI with ilv and could not be separated from dnaAts46 by transduction.
分离出了大肠杆菌dnaAts46的一个耐热突变体，它仅在高于39摄氏度的温度下才能正常生长。在从42摄氏度转移到32摄氏度后，相对于蛋白质，该突变体过量产生DNA。如杂交和其他实验所示，这是由于在低温下染色体复制轮次的过度起始所致。该突变由PI与ilv共转导，并且不能通过转导与dnaAts46分离。
