Katayama T, Takata M, Sekimizu K
Department of Microbiology, Kyushu University Faculty of Pharmaceutical Sciences, Fukuoka, Japan.
J Bacteriol. 1996 Oct;178(19):5790-2. doi: 10.1128/jb.178.19.5790-5792.1996.
Growth inhibition of the dnaA(Cs) mutant, which overinitiates chromosome replication, was shown to be dependent upon the nucleoid protein H-NS. [3H]thymine incorporation experiments indicated that the absence of H-NS inhibited overreplication by the dnaA(Cs) mutant. In addition, the temperature-sensitive phenotype of a dnaA46 mutant was enhanced by disruption of H-NS. These observations suggest that H-NS directly or indirectly facilitates the initiation of chromosome replication.
dnaA（Cs）突变体过度起始染色体复制，其生长抑制被证明依赖于类核蛋白H-NS。[3H]胸腺嘧啶掺入实验表明，H-NS的缺失抑制了dnaA（Cs）突变体的过度复制。此外，H-NS的缺失增强了dnaA46突变体的温度敏感表型。这些观察结果表明，H-NS直接或间接促进染色体复制的起始。
