Virology Department, Animal and Plant Health Agency (APHA)-Weybridge, Addlestone, Surrey, UK.
APHA-Preston, Animal Health Centre, Barton, Preston, UK.
Transbound Emerg Dis. 2022 Jul;69(4):1698-1706. doi: 10.1111/tbed.14539. Epub 2022 Apr 12.
Border disease (BD) was first reported in 1959 in lambs from the border region of England and Wales. The causative virus (BD virus; BDV) has since been identified in several other ruminant species and pigs. The virus is prevalent in sheep flocks of UK, Europe and USA and has potential to inflict substantial economic losses. Natural BDV infection of pigs was first reported in the UK in 1992 from pigs with haemorrhagic lesions and more recently from healthy pigs in Spain and Japan. Here, a persistent problem of poor growth and anaemia in a small proportion of growing pigs on a mixed pig and sheep holding was investigated and tissues were tested in a pan viral microarray. The microarray detected BDV RNA in several tissues which was further confirmed by sequencing, specific BDV PCR and immunohistochemistry. Phylogenetically, the virus clustered with other BDVs in the sub-genotype 1b. This investigation highlights likely interspecies transmission of pestiviruses and their impact on pestivirus detection and eradication programs.
边虫病（BD）于 1959 年首次在英格兰和威尔士边境地区的羔羊中报告。此后，已在其他几种反刍动物和猪中发现了致病病毒（BD 病毒；BDV）。该病毒在英国、欧洲和美国的绵羊群中流行，有造成重大经济损失的潜力。1992 年，英国首次报道了猪自然感染 BDV，当时猪出现出血性病变，最近在西班牙和日本的健康猪中也报道了猪自然感染 BDV。在此，对一个混合饲养猪和羊的小型养猪场中少数生长猪出现生长不良和贫血的持续问题进行了调查，并在全病毒微阵列中对组织进行了检测。微阵列在几种组织中检测到 BDV RNA，进一步通过测序、特定 BDV PCR 和免疫组织化学证实了这一点。从系统发育上看，该病毒与亚基因型 1b 中的其他 BDV 聚类。这项调查强调了瘟病毒的种间传播及其对瘟病毒检测和根除计划的影响。
