Department of Ophthalmology, University of Campinas, Campinas, São Paulo, Brazil.
J Glaucoma. 2022 Jul 1;31(7):567-573. doi: 10.1097/IJG.0000000000002026. Epub 2022 Mar 29.
The Laguna ONhE, a software that measures the hemoglobin (Hb) concentration of the optic nerve head (ONH) from fundus photographs, demonstrated good accuracy in discriminating healthy eyes from eyes with mild glaucoma.
The aim was to evaluate Hb concentration of the optic nerve to distinguish between healthy eyes and eyes with mild glaucoma.
Eyes from patients with mild primary open angle glaucoma (MD > -6 dB) (n=58) and from healthy subjects (n=64) were selected. Retinal nerve fiber layer thickness measurements of all eyes were acquired with optical coherence tomography. Optic disc photographs were also obtained, and the images were analyzed using the Laguna ONhE software, which measures the amount of Hb in 24 sectors of the ONH. The software also calculates the Glaucoma Discriminant Function (GDF), an index that expresses the chance of the ONH being compatible with glaucoma. Areas under the receiver operating characteristic curve and sensitivities at fixed specificities of 90% and 95% of each Laguna ONhE parameter were calculated.
The mean retinal nerve fiber layer thickness and vertical cup/disc ratio of the control and glaucoma groups were 90.0±10.6 μm versus 66.28±9.85 μm ( P <0.001) and 0.5±0.09 versus 0.65±0.09 ( P <0.001), respectively. Total Hb (67.9±4.45 vs. 62.89±4.89, P <0.001) and GDF (11.57±15.34 vs. -27.67±20.94, P <0.001) were significantly higher in the control group. The Hb concentration was also significantly higher in 21 of the 24 sectors in the control group compared with the glaucoma group ( P <0.05). The GDF had the largest areas under the receiver operating characteristic curve (0.93), with 79.3% sensitivity at a fixed specificity of 95%.
Measurements of optic nerve Hb concentration using a colorimetry photographic device demonstrated good accuracy in discriminating healthy eyes from eyes with mild glaucoma. Further studies are need to understand vascular factors implicated in the development of glaucoma.
Laguna ONhE 是一款软件，可从眼底照片中测量视神经头（ONH）的血红蛋白（Hb）浓度，该软件在区分健康眼与轻度青光眼眼方面具有良好的准确性。
旨在评估视神经 Hb 浓度以区分健康眼与轻度青光眼眼。
选择患有轻度原发性开角型青光眼（MD > -6dB）的患者（n=58）和健康受试者（n=64）的眼睛。所有眼睛的视网膜神经纤维层厚度测量均使用光学相干断层扫描获得。还获得视神经盘照片，并使用 Laguna ONhE 软件对图像进行分析，该软件可测量 ONH 24 个扇区的 Hb 量。该软件还计算青光眼判别函数（GDF），该指数表示 ONH 与青光眼相符的可能性。计算每个 Laguna ONhE 参数的 90%和 95%固定特异性的接收者操作特性曲线下面积和灵敏度。
对照组和青光眼组的平均视网膜神经纤维层厚度和垂直杯/盘比分别为 90.0±10.6μm 与 66.28±9.85μm（P<0.001）和 0.5±0.09 与 0.65±0.09（P<0.001）。对照组的总 Hb（67.9±4.45 vs. 62.89±4.89，P<0.001）和 GDF（11.57±15.34 vs. -27.67±20.94，P<0.001）均显著升高。与青光眼组相比，对照组的 24 个扇区中有 21 个的 Hb 浓度也显著升高（P<0.05）。GDF 的接收者操作特性曲线下面积最大（0.93），在固定特异性为 95%时，灵敏度为 79.3%。
使用比色照相设备测量视神经 Hb 浓度在区分健康眼与轻度青光眼眼方面具有良好的准确性。需要进一步研究以了解青光眼发展中涉及的血管因素。
