Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea; Department of Crop Science, Chungnam National University, Daejeon 34134, South Korea.
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, South Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, South Korea.
Cell Rep. 2022 Mar 29;38(13):110579. doi: 10.1016/j.celrep.2022.110579.
Tuberization is an important developmental process in potatoes, but it is highly affected by environmental conditions. Temperature is a major environmental factor affecting tuberization, with high temperatures suppressing tuber development. However, the temporal aspects of thermo-responsive tuberization remain elusive. In this study, we show that FT homolog StSP6A is suppressed by temporally distinct regulatory pathways. Experiments using StSP6A-overexpressing plants show that post-transcriptional regulation plays a major role at the early stage, while transcriptional regulation is an important late-stage factor, suppressing StSP6A at high temperatures in leaves. Overexpression of StSP6A in leaves restores tuber formation but does not recover tuber yield at the late stage, possibly because of suppressed sugar transport at high temperatures. Transcriptome analyses lead to the identification of potential regulators that may be involved in thermo-responsive tuberization at different stages. Our work shows that potato has temporally distinct molecular mechanisms that finely control tuber development at high temperatures.
块茎形成是马铃薯发育过程中的一个重要阶段，但它极易受到环境条件的影响。温度是影响块茎形成的主要环境因素，高温会抑制块茎的发育。然而，热响应性块茎形成的时间方面仍然难以捉摸。在这项研究中，我们表明，FT 同源物 StSP6A 受到时间上不同的调控途径的抑制。使用 StSP6A 过表达植物的实验表明，转录后调控在早期阶段起主要作用，而转录调控是一个重要的晚期因素，在叶片中高温下抑制 StSP6A。在叶片中过表达 StSP6A 可以恢复块茎的形成，但在晚期不会恢复块茎的产量，这可能是由于高温下糖的运输受到抑制。转录组分析导致鉴定出可能参与不同阶段热响应性块茎形成的潜在调节剂。我们的工作表明，马铃薯具有时间上不同的分子机制，可以精细地控制高温下的块茎发育。
