Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering & Technology Research Center of Coarse Cereal Industralization, College of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, China.
Key Laboratory of Wheat Crop Research in Ganzi Academy of Agricultural Sciences, Ganzi Academy of Agricultural Sciences, Ganzi, Sichuan, China.
PeerJ. 2024 Mar 26;12:e17183. doi: 10.7717/peerj.17183. eCollection 2024.
(phosphatidyl ethanolamine-binding protein) is widely found in eukaryotes including plants, animals and microorganisms. In plants, the family plays vital roles in regulating flowering time and morphogenesis and is highly associated to agronomic traits and yields of crops, which has been identified and characterized in many plant species but not well studied in Tartary buckwheat ( Gaertn.), an important coarse food grain with medicinal value.
Genome-wide analysis of gene family members in Tartary buckwheat was performed using bioinformatic tools. Subcellular localization analysis was performed by confocal microscopy. The expression levels of these genes in leaf and inflorescence samples were analyzed using qRT-PCR.
Fourteen () genes were identified and divided into three sub-clades according to their phylogenetic relationships. Subcellular localization analysis of the FtPEBP proteins in tobacco leaves indicated that FT- and TFL-GFP fusion proteins were localized in both the nucleus and cytoplasm. Gene structure analysis showed that most genes contain four exons and three introns. genes are unevenly distributed in Tartary buckwheat chromosomes. Three tandem repeats were found among /, / and /. Five orthologous gene pairs were detected between and . Seven light-responsive, nine hormone-related and four stress-responsive elements were detected in promoters. We used real-time PCR to investigate the expression levels of s among two flowering-type cultivars at floral transition time. We found / were highly expressed in leaf and young inflorescence of early-flowering type, whereas they were expressed at very low levels in late-flowering type cultivars. Thus, we deduced that / may be positive regulators for flowering and yield of Tartary buckwheat. These results lay an important foundation for further studies on the functions of genes which may be utilized for yield improvement.
（磷酸乙醇胺结合蛋白）广泛存在于真核生物中，包括植物、动物和微生物。在植物中，该家族在调控开花时间和形态发生方面发挥着重要作用，与作物的农艺性状和产量高度相关，已在许多植物物种中得到鉴定和特征描述，但在重要的粗粮作物苦荞（Gaertn.）中研究甚少，苦荞具有药用价值。
使用生物信息学工具对苦荞基因家族成员进行全基因组分析。通过共聚焦显微镜进行亚细胞定位分析。使用 qRT-PCR 分析这些基因在叶片和花序样品中的表达水平。
鉴定出 14 个（）基因，并根据系统发育关系分为三个亚簇。FtPEBP 蛋白在烟草叶片中的亚细胞定位分析表明，FT-和 TFL-GFP 融合蛋白定位于细胞核和细胞质。基因结构分析表明，大多数基因包含四个外显子和三个内含子。基因在苦荞染色体上不均匀分布。在 /、/ 和 / 之间发现了三个串联重复。在 和 之间检测到五个直系同源基因对。在启动子中检测到 7 个光响应、9 个激素相关和 4 个应激响应元件。我们使用实时 PCR 研究了两个开花型品种在花转变时间的表达水平。我们发现 / 在早花型的叶片和幼花序中高度表达，而在晚花型品种中表达水平很低。因此，我们推断 / 可能是苦荞开花和产量的正调控因子。这些结果为进一步研究 基因的功能奠定了重要基础，这些基因可能被用于提高产量。
