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用人白细胞培养物对间接诱变剂（环磷酰胺）进行体外试验：通过肝脏灌注和与粗制肝匀浆孵育实现激活。

Testing in vitro of an indirect mutagen (cyclophosphamide) with human leukocyte cultures: Activation by liver perfusion and by incubation with crude liver homogenate.

作者信息

Madle S, Westphal D, Hilbig V, Obe G

出版信息

Mutat Res. 1978 Aug;54(1):95-9. doi: 10.1016/0165-1161(78)90139-5.

DOI:10.1016/0165-1161(78)90139-5
PMID:353547
Abstract

The indirect mutagen cyclophosphamide was tested in human whole blood cultures with respect to chromosome-breaking activities and suppression of mitotic indices after activation by liver perfusion and crude liver homogenates with and without cofactors. Both methods produced nearly the same effects, with the exception of liver homogenate without cofactors which had only weak metabolic activities.

摘要

间接诱变剂环磷酰胺在人全血培养物中进行了测试，以研究其在经肝脏灌注以及添加和不添加辅助因子的粗制肝脏匀浆激活后的染色体断裂活性和有丝分裂指数抑制情况。除了不添加辅助因子的肝脏匀浆代谢活性较弱外，两种方法产生的效果几乎相同。

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Testing in vitro of an indirect mutagen (cyclophosphamide) with human leukocyte cultures: Activation by liver perfusion and by incubation with crude liver homogenate.用人白细胞培养物对间接诱变剂（环磷酰胺）进行体外试验：通过肝脏灌注和与粗制肝匀浆孵育实现激活。
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