Department of Surgery and Obstetric/Theriogenology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.
Arch Razi Inst. 2021 Nov 30;76(5):1519-1535. doi: 10.22092/ari.2021.355554.1697. eCollection 2021 Nov.
This study aimed to investigate the effect of the administration of powdered as a dietary supplement on testosterone concentration, spermatogenesis stimulating hormone, interstitial cell-stimulating hormone levels, sperms morphological characteristics, testis histological traits, and testicular size in the breeding and non-breeding season in adult rams. In total, 20 adult rams were included and randomly divided into three groups. The first group of adult rams (n=8) was subdivided into two subgroups of four rams (Sub-G1-B and Sub-G2-B). TheSub-G1-B and Sub-G2-B were fed 2 and 5 g of once a day, respectively, for 90 days during the breeding season. The second group of adult rams (n=8) was subdivided into two subgroups of four rams (Sub-G1-NB and Sub-G2-NB). The Sub-G1-NB and Sub-G2-NB were fed 2 and 5 g of once a day, respectively, for 90 days during the non-breeding season. The third group of adult rams (n=4) was considered the control group two times (in and out of the season). The results showed that the testosterone concentration and gonad protective and interstitial cell-stimulating hormone levels increased significantly (P<0.05) in both the experimental groups that received ginseng supplementation, compared to the control group in and out of the breeding season. The evaluation of sperm morphometric parameters, such as total sperm count, total motility, and progressive motility, showed superiority in improving the above-mentioned parameters. However, the total immotile sperms and non-progressive sperms underwent a significant decrease (P<0.05) in both experimental groups of ginseng supplemented, compared to the control group in and out of season. The angiogenesis of the seminiferous tubules increased significantly (P<0.05) in both experimental groups. Through a microscopic examination, the recorded data showed a significant increase in the population of spermatogonial stem cells as well as primary and secondary spermatocytes in both experimental groups. Values of testicular diameter showed a significant increase (P<0.05) after a period of 75 and 90 days following the initiation of treatments in both experimental groups, compared to the control group in and out of the season. It can be concluded that has some beneficial effects on the antioxidant status of the semen, the morphometric parameters, and other critical traits of sperm and testicles which are the important factors in male fertility.
本研究旨在探讨作为膳食补充剂给药对繁殖和非繁殖季节成年公羊的睾丸中睾酮浓度、促精子生成素、间质细胞刺激素水平、精子形态特征、睾丸组织学特征和睾丸大小的影响。共纳入 20 只成年公羊，并随机分为三组。第一组成年公羊（n=8）分为两组，每组 4 只公羊（Sub-G1-B 和 Sub-G2-B）。Sub-G1-B 和 Sub-G2-B 在繁殖季节每天分别口服 2 和 5 克 90 天。第二组成年公羊（n=8）分为两组，每组 4 只公羊（Sub-G1-NB 和 Sub-G2-NB）。Sub-G1-NB 和 Sub-G2-NB 在非繁殖季节每天分别口服 2 和 5 克 90 天。第三组成年公羊（n=4）被认为是对照组两次（进出季节）。结果表明，与繁殖季节和非繁殖季节的对照组相比，接受人参补充的两组实验动物的睾酮浓度、性腺保护和间质细胞刺激素水平显著升高（P<0.05）。精子形态计量学参数，如总精子数、总活力和前向运动，在提高上述参数方面表现出优越性。然而，与繁殖季节和非繁殖季节的对照组相比，接受人参补充的两组实验动物的总不动精子和非前向运动精子显著减少（P<0.05）。生精小管的血管生成显著增加（P<0.05）。通过显微镜检查，记录的数据显示，两组实验动物的精原干细胞以及初级和次级精母细胞的数量均显著增加。两组实验动物的睾丸直径在治疗开始后 75 天和 90 天后均显著增加（P<0.05），与繁殖季节和非繁殖季节的对照组相比。可以得出结论， 对精液的抗氧化状态、形态计量学参数以及精子和睾丸的其他关键特征具有一些有益的影响，这些特征是雄性生育能力的重要因素。
