Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad, Iraq.
Arch Razi Inst. 2021 Nov 30;76(5):1307-1314. doi: 10.22092/ari.2021.356104.1778. eCollection 2021 Nov.
The spread of different types of cancer has been on a rise in the recent century. The use of chemical medications develops drug resistance and causes serious side effects. () is one of the most famous herbal remedies considered owing to its anti-inflammatory effect and boosting the intimate immune system; moreover, it has demonstrated some anti-proliferative effects on cancerous cell lines. The current study assessed the cytotoxic activity of alcoholic extract of () at different concentrations of 6.125, 12.5, 25, 50, and 100 µg/mL on breast cancer cell line MCF-7. The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS)) analyses were employed to study the antioxidant efficiency of ethanolic extract of . The results of Fourier transmission infra-red analysis and Phytochemical screening pointed to the presence of many active compounds in this extract, such as Tannins, Saponins, Phenolic compounds, Reducing sugar, Terpenoids, Steroids, Glucosides, Alkaloids, and Flavonoids. Furthermore, the results demonstrated that this extract significantly inhibited the growth of the MCF-7 cell line in a concentration-dependent manner, as compared to the control, and the cytotoxic activity of this extract elevated with an increase in the concentration. The results pointed out that can be considered a particularly valuable source of effective anti-proliferative and cytotoxic agents. The experimental findings demonstrated that extract possessed significant antioxidant efficiency and anti-proliferative effects on cancerous cell lines.
在最近一个世纪，不同类型的癌症的发病率一直在上升。化学药物的使用会产生抗药性并导致严重的副作用。()是最著名的草药之一，因其抗炎作用和增强免疫系统的功能而备受推崇；此外，它已显示出对癌细胞系的一些抗增殖作用。本研究评估了不同浓度（6.125、12.5、25、50 和 100 µg/mL）的()的醇提物对乳腺癌细胞 MCF-7 的细胞毒性活性。采用 2,2-二苯基-1-苦基肼水合物（DPPH）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）分析来研究醇提物的抗氧化效率。傅里叶变换红外分析和植物化学筛选的结果表明，这种提取物中存在许多活性化合物，如单宁、皂苷、酚类化合物、还原糖、萜类化合物、甾体、糖苷、生物碱和类黄酮。此外，结果表明，与对照组相比，该提取物以浓度依赖的方式显著抑制 MCF-7 细胞系的生长，并且随着浓度的增加，该提取物的细胞毒性活性也升高。结果表明，可以将()视为具有有效抗增殖和细胞毒性作用的特别有价值的来源。实验结果表明，()提取物具有显著的抗氧化效率和对癌细胞系的抗增殖作用。
