Division of Infectious Diseases, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Excellence Research Laboratory on Natural Products, Division of Biological Science, Faculty of Science and Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Excellence Research Laboratory on Natural Products, Division of Biological Science, Faculty of Science and Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Food Chem. 2021 May 15;344:128571. doi: 10.1016/j.foodchem.2020.128571. Epub 2020 Nov 5.
Extract of E. camaldulensis was partitioned into aqueous and ethanol fractions by a precipitation and sedimentation-based technique and profiled for phytochemical components. Antimicrobial evaluation yielded inhibitory concentrations of 16-64 and 158-316 µg/mL, and bactericidal concentrations of 32-64 and 316->2528 µg/mL for ethanol and aqueous fractions, respectively. Antioxidant activities evaluated using 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic) acid assays showed IC values of 7.07 and 65.67 µg/mL, and 17.96 and 201.3 µg/mL for aqueous and ethanol fractions. Total phenolic content of 9.04 ± 0.26 and 3.58 ± 0.04 GAE/mg fraction, and flavonoid content of 2.07 ± 0.02 and 3.37 ± 0.05 QE/mg fraction were recorded for aqueous and ethanol fractions. At subinhibitory concentrations fractions significantly reduced listeriolysin O-induced haemolysis (p < 0.05), and ameliorated HO-induced toxicity by 8-23 and 15-83%. Nitrite production reduced by 4-17 and 3-14 µM following fractions treatment. The fractions showed bioactive properties, with oxidative stress amelioratory effects, and could be a potentials source of preservatives and functional food additives.
从埃及无花果树中提取的物质，通过沉淀和沉降技术分为水相和乙醇相，分析其化学成分。抗菌评估结果显示，乙醇相和水相的抑菌浓度分别为 16-64 和 158-316µg/mL，杀菌浓度分别为 32-64 和 316->2528µg/mL。使用 2,2-二苯基-1-苦基肼基（DPPH）和 2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）两种方法评估抗氧化活性，结果显示水相和乙醇相的 IC 值分别为 7.07 和 65.67µg/mL，以及 17.96 和 201.3µg/mL。水相和乙醇相的总酚含量分别为 9.04±0.26 和 3.58±0.04 GAE/mg 分数，黄酮含量分别为 2.07±0.02 和 3.37±0.05 QE/mg 分数。在亚抑菌浓度下，各分数可显著降低李斯特菌溶血素 O 诱导的溶血（p<0.05），并将 HO 诱导的毒性降低 8-23%和 15-83%。经各分数处理后，亚硝酸盐的产生量分别减少了 4-17%和 3-14%。这些分数表现出生物活性，具有改善氧化应激的作用，可能是防腐剂和功能性食品添加剂的潜在来源。
