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桉属植物叶提取物的体外抗菌、抗真菌和抗氧化活性与酚类成分的关系。

In vitro antibacterial, antifungal and antioxidant activities of Eucalyptus spp. leaf extracts related to phenolic composition.

作者信息

Elansary Hosam O, Salem Mohamed Z M, Ashmawy Nader A, Yessoufou Kowiyou, El-Settawy Ahmed A A

机构信息

a Medicinal Plants Laboratory, Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby) , Alexandria University , Alexandria , Egypt.

b Department of Forestry and Wood Technology, Faculty of Agriculture (El-Shatby) , Alexandria University , Alexandria , Egypt.

出版信息

Nat Prod Res. 2017 Dec;31(24):2927-2930. doi: 10.1080/14786419.2017.1303698. Epub 2017 Mar 16.

DOI:10.1080/14786419.2017.1303698
PMID:28299978
Abstract

The crude methanolic extracts from leaves of Eucalyptus camaldulensis L., E. camaldulensis var obtusa and E. gomphocephala grown in Egypt were investigated to explore their chemical composition as well as their antibacterial, antifungal and antioxidant activities. Major phenolics found were ellagic acid, quercetin 3-O-rhamnoside, quercetin 3-O-b-D-glucuronide, caffeic acid and chlorogenic acid. The antioxidant activities were examined by the 2,2'-diphenypicrylhydrazyl (DPPH) and β-Carotene-linoleic acid assays. E. camaldulensis extracts showed the highest phenolic content, antioxidant and antimicrobial activities compared to other cultivars. MIC values reported for antibacterial activity of E. camaldulensis ranged from 0.08 μg/mL (Bacillus cereus) to 0.22 μg/mL (Staphylococcus aureus), while MBC values ranged from 0.16 μg/mL (Dickeya solani and B. cereus) to 0.40 μg/mL (S. aureus). The inhibitory activities against growth of bacteria and fungi used is an indication that E. camaldulensis a might be useful resource for the development and formulation of antibacterial and antifungal drugs.

摘要

对生长在埃及的赤桉、钝叶赤桉和大头桉树叶的粗甲醇提取物进行了研究,以探索其化学成分以及抗菌、抗真菌和抗氧化活性。发现的主要酚类物质有鞣花酸、槲皮素3-O-鼠李糖苷、槲皮素3-O-β-D-葡萄糖醛酸苷、咖啡酸和绿原酸。通过2,2'-二苯基苦味酰基自由基(DPPH)和β-胡萝卜素-亚油酸测定法检测抗氧化活性。与其他品种相比,赤桉提取物表现出最高的酚类含量、抗氧化和抗菌活性。报道的赤桉抗菌活性的最低抑菌浓度(MIC)值范围为0.08μg/mL(蜡样芽孢杆菌)至0.22μg/mL(金黄色葡萄球菌),而最低杀菌浓度(MBC)值范围为0.16μg/mL(胡萝卜软腐果胶杆菌和蜡样芽孢杆菌)至0.40μg/mL(金黄色葡萄球菌)。对所用细菌和真菌生长的抑制活性表明,赤桉可能是开发和配制抗菌和抗真菌药物的有用资源。

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