Yamamoto Yuko, Tabata Kazufumi
New Field Pioneering Division Toyota Boshoku Corporation Kariya Japan.
Toyota Boshoku Europe N.V. Zaventem Belgium.
Plant Direct. 2022 Mar 27;6(3):e391. doi: 10.1002/pld3.391. eCollection 2022 Mar.
Plant yield can be increased by matching the internal circadian rhythms with the external light and dark cycle (circadian resonance). The circadian resonance reported in the past was analyzed under light-dark cycles with 20-, 24-, or 28-hr periods; however, the mechanism for circadian resonance is still debatable due to the experimental time schedules in previous studies being few in number and widely separated. By analyzing the yield of grown under eight different external light and dark periods, we found that the yield increased when the external cycle was 22 and 26 hr instead of 24 hr. Time course RNA-seq analysis determined that seedling circadian clock genes had a free-running period of 22 ± 1 hr. Furthermore, a group of genes with 25- to 26-hr period rhythms were also observed in the seedlings with a 22- ± 1-hr period as their circadian clock. We propose that resonance that occurred by matching the expression time of a group of genes with the 25- to 26-hr cycle and providing an external day-night cycle of 25 to 26 hr was one factor that caused the yield increase.
通过使内部昼夜节律与外部明暗周期相匹配（昼夜节律共振），可以提高作物产量。过去报道的昼夜节律共振是在周期为20小时、24小时或28小时的明暗周期下进行分析的；然而，由于先前研究中的实验时间安排数量较少且间隔较大，昼夜节律共振的机制仍存在争议。通过分析在八种不同外部明暗周期下生长的作物产量，我们发现当外部周期为22小时和26小时而非24小时时，产量会增加。时间进程RNA测序分析确定，幼苗昼夜节律时钟基因的自由运行周期为22±1小时。此外，在以22±1小时为昼夜节律时钟周期的幼苗中，还观察到一组周期为25至26小时的节律基因。我们提出，通过使一组基因的表达时间与25至26小时的周期相匹配，并提供25至26小时的外部昼夜周期而发生的共振是导致产量增加的一个因素。
