ICAR National Professor Project, ICAR-Indian Institute of Rice Research, Hyderabad, 500030, Telangana, India.
Department of Plant Breeding and Genetics, ICAR-Indian Institute of Rice Research, Hyderabad, 500030, Telangana, India.
Planta. 2022 Sep 7;256(4):71. doi: 10.1007/s00425-022-03983-3.
Identification of trait enhancing QTLs for yield and photosynthesis-related traits in rice using interspecific mapping population and chromosome segment substitution lines derived from a cross between Oryza sativa and Oryza rufipogon. Wild rice contains novel genes which can help in improving rice yield. Common wild rice Oryza rufipogon is a known source for enhanced photosynthesis and yield-related traits. We developed BCF mapping populations using O. rufipogon IC309814 with high photosynthetic rate as donor, and elite cultivar MTU1010 as recurrent parent. Evaluation of 238 BCF families for 13 yield-related traits and 208 BCF families for seven photosynthesis-related physiological traits resulted in identification of significantly different lines which performed better than MTU1010 for various yield contributing traits. 49 QTLs were identified for 13 yield traits and 7 QTLs for photosynthesis-related traits in BCF In addition, 34 QTLs in BCF and 26 QTLs in BCF were also detected for yield traits.11 common QTLs were identified in three consecutive generations and their trait-increasing alleles were derived from O. rufipogon. Significantly, one major effect common QTL qTGW3.1 for thousand grain weight with average phenotypic variance 8.1% and one novel QTL qBM7.1 for biomass were identified. Photosynthesis-related QTLs qP9.1, qP12.1, qP12.2 qSPAD1.1 and qSPAD6.1 showed additive effect from O. rufipogon. A set of 145 CSSLs were identified in BCF which together represented 87% of O. rufipogon genome. In addition, 87 of the 145 CSSLs were significantly different than MTU1010 for at least one trait. The major effect QTLs can be fine mapped for gene discovery. CSSLs developed in this study are a good source of novel alleles from O. rufipogon in the background of Cottondora Sannalu for rapid improvement of any trait in rice.
利用籼稻/野生稻杂交群体和染色体片段代换系鉴定水稻产量和光合作用相关性状的增强 QTL。野生稻含有可提高水稻产量的新基因。普通野生稻 Oryza rufipogon 是提高光合作用和产量相关性状的已知来源。我们以光合速率较高的野生稻 IC309814 为供体，以优良品种 MTU1010 为轮回亲本，构建了 BCF 作图群体。对 238 个 BCF 家系的 13 个产量相关性状和 208 个 BCF 家系的 7 个光合作用相关生理性状进行了评价，结果鉴定出了一些表现明显优于 MTU1010 的显著差异系，这些系在各种产量性状上表现更好。在 BCF 中鉴定到 49 个产量性状 QTL 和 7 个光合作用相关性状 QTL。此外，在 BCF 中还检测到 34 个产量性状 QTL 和 26 个产量性状 QTL。在连续三代中鉴定到 11 个共同 QTL，它们的增效等位基因来自 O. rufipogon。值得注意的是，鉴定到一个控制千粒重的主效共同 QTL qTGW3.1，表型方差为 8.1%，以及一个控制生物量的新 QTL qBM7.1。光合作用相关 QTL qP9.1、qP12.1、qP12.2、qSPAD1.1 和 qSPAD6.1 表现出来自 O. rufipogon 的加性效应。在 BCF 中鉴定到 145 个 CSSL，它们共同代表了 87%的 O. rufipogon 基因组。此外，在 145 个 CSSL 中有 87 个在至少一个性状上与 MTU1010 有显著差异。这些主效 QTL 可以进行精细定位，用于基因发现。本研究中开发的 CSSL 是从 O. rufipogon 背景中快速改良棉花品种任何性状的新型等位基因的良好来源。
