Kim Sang-Hoon, Ko Il-Gyu, Jin Jun-Jang, Hwang Lakkyong, Yoon Hye-Sun, Baek Seung-Soo
Department of Sport & Health Sciences, College of Art & Culture, Sangmyung University, Seoul, Korea.
Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
J Exerc Rehabil. 2022 Feb 24;18(1):28-33. doi: 10.12965/jer.2244002.001. eCollection 2022 Feb.
We reported that application of ethanol with lipopolysaccharide (LPS) and carbon tetrachloride (CCl) enhanced alanine aminotransferase (ALT) and aspartate aminotransferase (AST) level. In the current experiment, the protective effect of treadmill running on liver injury caused by ethanol with LPS and CCl in mice was studied. Liver injury severity was determined by measuring ALT and AST level in the blood. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, immunohistochemistry for caspase-3, and Western blotting for Bcl-2-associated X protein (Bax) and B-cell lymphoma 2 (Bcl-2) were performed to indicate hepatocyte apoptosis. In addition, to understand the mechanism, 5'-adenosine monophosphate-activated protein kinase (AMPK) phosphorylation was studied by Western blotting. Treadmill exercise ameliorated ethanol with LPS and CCl-mediated elevation of ALT and AST level. Treadmill exercise suppressed ethanol with LPS and CCl-mediated elevation of the TUNEL-positive cell number and cleaved caspase-3 expression. Treadmill exercise suppressed ethanol with LPS and CCl-mediated elevation of Bax expression and increased Bcl-2 expression suppressed by application of ethanol with LPS and CCl. Treadmill exercise enhanced AMPK phosphorylation which was suppressed by application of ethanol with LPS and CCl. Treadmill exercise has the effect of reducing liver damage caused by alcohol and or drug addiction.
我们报道,乙醇与脂多糖(LPS)和四氯化碳(CCl)联合应用会提高丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平。在当前实验中,研究了跑步机跑步对乙醇与LPS和CCl所致小鼠肝损伤的保护作用。通过检测血液中的ALT和AST水平来确定肝损伤的严重程度。进行末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TUNEL)染色、半胱天冬酶-3的免疫组织化学检测以及Bcl-2相关X蛋白(Bax)和B细胞淋巴瘤2(Bcl-2)的蛋白质印迹分析以指示肝细胞凋亡。此外,为了解其机制,通过蛋白质印迹分析研究了5'-腺苷单磷酸激活蛋白激酶(AMPK)的磷酸化。跑步机运动改善了乙醇与LPS和CCl介导的ALT和AST水平升高。跑步机运动抑制了乙醇与LPS和CCl介导的TUNEL阳性细胞数量增加和半胱天冬酶-3裂解产物表达升高。跑步机运动抑制了乙醇与LPS和CCl介导的Bax表达升高,并增加了被乙醇与LPS和CCl抑制的Bcl-2表达。跑步机运动增强了被乙醇与LPS和CCl抑制的AMPK磷酸化。跑步机运动具有减轻由酒精和/或药物成瘾引起的肝损伤的作用。