Lee Seunghwan, Won Kyu Yeoun, Joo Sunhyung
Department of Surgery, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea.
Department of Pathology, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea.
Int Neurourol J. 2020 Nov;24(Suppl 2):88-95. doi: 10.5213/inj.2040430.215. Epub 2020 Nov 23.
Polydeoxyribonucleotide (PDRN) is a substance known to suppress inflammation and accelerate wound healing. In this experiment, the effect of PDRN treatment on carbon tetrachloride (CCl4)-evoked acute liver injury (ALI) was investigated using mice.
We analyzed the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and conducted hematoxylin and eosin staining in accompany with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining. Western blot analysis was also conducted to assess the expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, adenosine A2A receptor, Bcl-2-associated X protein (Bax), and B-cell lymphoma 2 (Bcl-2). The mice were received intraperitoneal injection of 10-mL/kg CCl4, 4 times, once every 2 days. The mice in the PDRN treatment groups received intraperitoneal injection of 200-μL distilled water comprising each concentration of PDRN for 7 days starting 1 day after first CCl4 injection.
ALT and AST concentrations in the serum were reduced and TNF-α, IL-1β, and IL-6 expressions were decreased by PDRN injection in CCl4-evoked ALI mice. PDRN injection suppressed Bax versus Bcl-2 ratio and reduced the percentage of TUNE-positive cells in CCl4-evoked ALI mice. PDRN injection overexpressed adenosine A2A receptor in CCl4-evoked ALI mice.
The therapeutic efficacy of PDRN also can be expected for CCl4-evoked acute urogenital injury in addition to ALI. The current research suggests that PDRN may be used for the therapeutic agent of CCl4-evoked ALI.
聚脱氧核糖核苷酸（PDRN）是一种已知可抑制炎症并加速伤口愈合的物质。在本实验中，使用小鼠研究了PDRN治疗对四氯化碳（CCl4）诱发的急性肝损伤（ALI）的影响。
我们分析了血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）的水平，并进行了苏木精和伊红染色以及末端脱氧核苷酸转移酶介导的dUTP缺口末端标记染色。还进行了蛋白质印迹分析，以评估肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、IL-6、腺苷A2A受体、Bcl-2相关X蛋白（Bax）和B细胞淋巴瘤2（Bcl-2）的表达。小鼠腹腔注射10 mL/kg CCl4，共4次，每2天注射一次。PDRN治疗组的小鼠在首次注射CCl4后1天开始，腹腔注射含各浓度PDRN的200 μL蒸馏水，持续7天。
在CCl4诱发的ALI小鼠中，注射PDRN可降低血清中ALT和AST的浓度，并降低TNF-α、IL-1β和IL-6的表达。注射PDRN可抑制CCl4诱发的ALI小鼠中Bax与Bcl-2的比例，并降低TUNEL阳性细胞的百分比。注射PDRN可使CCl4诱发的ALI小鼠中的腺苷A2A受体过表达。
除ALI外，PDRN对CCl4诱发的急性泌尿生殖系统损伤的治疗效果也值得期待。目前的研究表明，PDRN可用于CCl4诱发的ALI的治疗药物。
