Horiuchi M, Mochizuki M, Ishiguro N, Nagasawa H, Shinagawa M
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
J Vet Med Sci. 1997 Feb;59(2):133-6. doi: 10.1292/jvms.59.133.
To obtain monoclonal antibodies (MAbs) specific to feline panleukopenia virus (FPLV) and mink enteritis virus (MEV), 15 hybridomas secreting MAbs against MEV-Abashiri were established and the properties of the MAbs were analyzed. The cross-reactivity of MAbs revealed that one MAb, P2-215 was specific for FPLV and MEV, whereas the remaining fourteen MAbs reacted with canine parvovirus (CPV), FPLV, and MEV. Epitope analyses using various CPV/MEV chimeric viruses revealed that the MAb P2-215 recognized the epitope comprised of amino acid 93-Lys in VP2, which is known to be FPLV and MEV-specific.
为获得针对猫泛白细胞减少症病毒（FPLV）和水貂肠炎病毒（MEV）的单克隆抗体（MAb），建立了15株分泌抗MEV-阿巴什里单克隆抗体的杂交瘤，并对这些单克隆抗体的特性进行了分析。单克隆抗体的交叉反应性表明，一种单克隆抗体P2-215对FPLV和MEV具有特异性，而其余14种单克隆抗体与犬细小病毒（CPV）、FPLV和MEV发生反应。使用各种CPV/MEV嵌合病毒进行的表位分析表明，单克隆抗体P2-215识别由VP2中第93位赖氨酸组成的表位，该表位已知是FPLV和MEV特有的。
