Bendaoud Farah, Kim Gunjune, Larose Hailey, Westwood James H, Zermane Nadjia, Haak David C
Department of Botany Ecole Nationale Supérieure Agronomique, ENSA Algiers Algeria.
Department of Plant Pathology, Physiology and Weed Science Virginia Tech Blacksburg Virginia USA.
Ecol Evol. 2022 Mar 24;12(3):e8750. doi: 10.1002/ece3.8750. eCollection 2022 Mar.
Crenate broomrape ( Forsk.) is a serious long-standing parasitic weed problem in Algeria, mainly affecting legumes but also vegetable crops. Unresolved questions for parasitic weeds revolve around the extent to which these plants undergo local adaptation, especially with respect to host specialization, which would be expected to be a strong selective factor for obligate parasitic plants. In the present study, the genotyping-by-sequencing (GBS) approach was used to analyze genetic diversity and population structure of 10 Northern Algerian . populations with different geographical origins and host species (faba bean, pea, chickpea, carrot, and tomato). In total, 8004 high-quality single-nucleotide polymorphisms (5% missingness) were obtained and used across the study. Genetic diversity and relationships of 95 individuals from 10 populations were studied using model-based ancestry analysis, principal components analysis, discriminant analysis of principal components, and phylogeny approaches. The genetic differentiation ( ) between pairs of populations was lower between adjacent populations and higher between geographically separated ones, but no support was found for isolation by distance. Further analyses identified four genetic clusters and revealed evidence of structuring among populations and, although confounded with location, among hosts. In the clearest example, . growing on pea had a SNP profile that was distinct from other host/location combinations. These results illustrate the importance and potential of GBS to reveal the dynamics of parasitic weed dispersal and population structure.
锯齿列当（Forsk.）是阿尔及利亚长期存在的严重寄生杂草问题，主要影响豆类作物，但也会影响蔬菜作物。寄生杂草尚未解决的问题围绕着这些植物在多大程度上经历了局部适应，特别是在寄主专一性方面，这预计是专性寄生植物的一个强大选择因素。在本研究中，采用简化基因组测序（GBS）方法分析了来自阿尔及利亚北部10个具有不同地理起源和寄主物种（蚕豆、豌豆、鹰嘴豆、胡萝卜和番茄）的种群的遗传多样性和种群结构。总共获得了8004个高质量单核苷酸多态性（缺失率为5%）并在整个研究中使用。使用基于模型的祖先分析、主成分分析、主成分判别分析和系统发育方法研究了来自10个种群的95个个体的遗传多样性和关系。相邻种群之间的成对种群遗传分化（）较低，地理上分离的种群之间的遗传分化较高，但未发现距离隔离的证据。进一步分析确定了四个遗传簇，并揭示了种群之间以及尽管与地理位置混淆但寄主之间存在结构的证据。最明显的例子是，生长在豌豆上的锯齿列当具有与其他寄主/位置组合不同的单核苷酸多态性图谱。这些结果说明了GBS在揭示寄生杂草扩散动态和种群结构方面的重要性和潜力。
