Boukteb Amal, Sakaguchi Shota, Ichihashi Yasunori, Kharrat Mohamed, Nagano Atsushi J, Shirasu Ken, Bouhadida Mariem
Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia.
Field Crop Laboratory, National Institute of Agricultural Research of Tunisia, Carthage University, Tunis, Tunisia.
Front Plant Sci. 2021 Apr 13;12:618245. doi: 10.3389/fpls.2021.618245. eCollection 2021.
Poiret is a holoparasitic plant that lacks chlorophyll and totally depending on its host for its growth. parasitizes host plant roots and extract nutrient and water via a haustorium. Although distributes in the Mediterranean region as a wild plant parasite, it parasitizes faba bean causing serious damages which may reach 90% yield losses in Tunisia. Analysis of genetic diversity of the parasite is important to better understand its evolution and spread, remained largely unknown. In this work, we present the first study on genetic diversity and population structure using the robust technique Restriction-site-Associated DNA sequencing (RADseq) for spp. We collected 244 samples of from 18 faba bean fields in the north of Tunisia including 17 populations from the north-west and one population form the north-east. To overcome the difficulty of SNP discovery in genome as a non-model and tetraploid plant, we utilized three different informatics pipelines, namely UNEAK, pyRAD and Stacks. This study showed that genetic differentiation occurred in the Tunisian emphasizing the isolation by distance effect. However, no strong population clustering was detected in this work basing on the three data sets and clustering methods used. The present study shed the light on the current distribution and the genetic variation situation of the fetid broomrape in Tunisia, highlighting the importance of understanding the evolution of this parasite and its genetic background. This will aid in developing efficient strategies to control this parasite and its expansion in Tunisia and worldwide.
列当属植物是一种全寄生植物，缺乏叶绿素，完全依赖宿主生长。它寄生在宿主植物根部，通过吸器获取养分和水分。尽管作为野生植物寄生虫分布在地中海地区，但它寄生在蚕豆上会造成严重损害，在突尼斯可能导致高达90%的产量损失。分析该寄生虫的遗传多样性对于更好地理解其进化和传播很重要，但这在很大程度上仍不清楚。在这项工作中，我们首次使用强大的限制性位点关联DNA测序（RADseq）技术对列当属植物的遗传多样性和种群结构进行了研究。我们从突尼斯北部的18个蚕豆田收集了244个列当属植物样本，包括来自西北部的17个种群和来自东北部的1个种群。为了克服在非模式四倍体植物列当属基因组中发现单核苷酸多态性（SNP）的困难，我们使用了三种不同的信息学流程，即UNEAK、pyRAD和Stacks。这项研究表明，突尼斯的列当属植物存在遗传分化，强调了距离隔离效应。然而，基于所使用的三个数据集和聚类方法，在这项工作中未检测到强烈的种群聚类。本研究揭示了突尼斯臭列当的当前分布和遗传变异情况，突出了了解这种寄生虫进化及其遗传背景的重要性。这将有助于制定有效的策略来控制这种寄生虫及其在突尼斯和全球的扩散。
