Departamento de Sanidad Animal, Facultad de Veterinaria, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad de Murcia, Murcia, Spain.
Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic.
Transbound Emerg Dis. 2022 Sep;69(5):e1854-e1864. doi: 10.1111/tbed.14520. Epub 2022 Apr 6.
Antibodies against Phlebotomus perniciosus sandfly salivary gland homogenate (SGH) and recombinant protein rSP03B, sandfly-borne Toscana virus (TOSV), Sandfly Fever Sicilian virus (SFSV) and Leishmania, as well as DNA of the latter parasite, were investigated in 670 blood samples from 575 human donors in Murcia Region, southeast Spain, in 2017 and 2018. The estimated SGH and rSP03B seroprevalences were 69% and 88%, respectively, although correlation between test results was relatively low (ρ = 0.39). Similarly, TOSV, SFSV and Leishmania seroprevalences were 26%, 0% and 1%, respectively, and Leishmania PCR prevalence was 2%. Prevalences were significantly greater in 2017, overdispersed and not spatially related to each other although both were positively associated with SGH but not to rSP03B antibody optical densities, questioning the value of the latter as a diagnostic marker for these infections in humans.
在 2017 年和 2018 年，对来自西班牙东南部穆尔西亚地区 575 名人类供体的 670 份血液样本进行了针对白蛉唾液腺匀浆（SGH）和重组蛋白 rSP03B、白蛉传播的托斯卡纳病毒（TOSV）、西西里发热病毒（SFSV）和利什曼原虫以及后者寄生虫的 DNA 的抗体检测。估计的 SGH 和 rSP03B 血清阳性率分别为 69%和 88%，尽管测试结果之间的相关性相对较低（ρ=0.39）。同样，TOSV、SFSV 和利什曼原虫的血清阳性率分别为 26%、0%和 1%，而利什曼原虫 PCR 的阳性率为 2%。2017 年的患病率明显更高，呈过离散分布且彼此之间没有空间关系，尽管两者均与 SGH 呈正相关，但与 rSP03B 抗体光密度无关，这对后者作为这些人类感染的诊断标志物的价值提出了质疑。
