Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-food Processing, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China.
Hangzhou Botanical Garden, Hangzhou, 310007, P. R. China.
Food Funct. 2022 Apr 20;13(8):4612-4623. doi: 10.1039/d1fo03730g.
Cyanidin-3--glucoside (C3G), an essential representative of anthocyanins, has been proved to possess a myriad of biological activities. However, the effects of C3G on glucose metabolism and its underlying molecular mechanisms remain elusive. The aim of the present study was to investigate the metabolic impact of C3G on db/db mice and to determine whether its consequent anti-diabetic effects were related to glucose transporter-1 (GLUT-1) by and studies. As a result, through diabetic db/db mice, C3G treatment was found to significantly reduce the fasting blood glucose level and increase glycogen synthesis, which were associated with upregulation of GLUT-1 expression in the liver of the mice. In addition, in liver cells of the HepG2 and L02 lines, we further discovered that C3G could effectively promote glucose consumption by regulating the Wnt/β-catenin-WISP1 signaling pathway. Nevertheless, such effects would be restricted when the expression of GLUT-1 was blocked by the inhibitor IWR-1. Meanwhile, molecular docking technology was applied to simulate the possible action sites of C3G at the molecular level, and the results indicated that C3G might bind to β-catenin. In conclusion, our study provided evidence of the antihyperglycemic effect of C3G and regulating GLUT-1 expression and the related signaling pathways.
矢车菊素-3-葡萄糖苷（C3G）作为花色苷的重要代表，已被证明具有多种生物学活性。然而，C3G 对葡萄糖代谢的影响及其潜在的分子机制仍不清楚。本研究旨在探讨 C3G 对 db/db 小鼠代谢的影响，并通过 和 研究确定其抗糖尿病作用是否与葡萄糖转运蛋白-1（GLUT-1）有关。结果表明，通过糖尿病 db/db 小鼠，C3G 处理可显著降低空腹血糖水平并增加肝糖原合成，这与小鼠肝脏中 GLUT-1 表达上调有关。此外，在 HepG2 和 L02 细胞系的肝细胞中，我们进一步发现 C3G 可以通过调节 Wnt/β-catenin-WISP1 信号通路有效促进葡萄糖消耗。然而，当用抑制剂 IWR-1 阻断 GLUT-1 的表达时，这种作用会受到限制。同时，应用分子对接技术在分子水平上模拟 C3G 的可能作用部位，结果表明 C3G 可能与 β-catenin 结合。总之，本研究为 C3G 的降血糖作用及其调节 GLUT-1 表达和相关信号通路提供了证据。
