Department of Agronomy, Bahauddin Zakariya University, Multan, Pakistan.
Department of Agronomy, College of Agriculture, University of Sargodha, Sargodha, Pakistan.
PLoS One. 2022 Mar 31;17(3):e0265956. doi: 10.1371/journal.pone.0265956. eCollection 2022.
Boron-deficiency in Yermosols is among the major constraints to mungbean productivity and grain biofortification in Pakistan. However, agronomic strategies such as boron (B) seed priming have potential to improve mungbean yield and grain biofortification. Moreover, deficiency to toxicity range for B is very narrow; therefore, it is pre-requisite to optimize its dose before field evaluation. A wire house experiment was planned out to reconnoiter the impact of seed priming with B on growth and quality of two cultivars of mungbean, i.e., 'NM-2011' and 'NM-2016'. Four different B levels were used as seed priming, i.e., 0.01%, 0.05%, 0.1% and 1.0% B, (borax Na2B4O7.10H2O, 11.5% B) were tested, whereas hydropriming was regarded as control. Seed priming with 0.01% B significantly (p≤0.05) lowered time taken to start germination and time to reach 50% emergence, whereas improved mean emergence time, emergence index, final emergence percentage, number of leaves, dry and fresh weight of root, shoot, and total weight, root length, plant height, chlorophyll contents, number of pods and 100-grain weight, seeds per plant, grain yield per plant, B concentrations in stem and grain, grain protein, carbohydrate and fiber in both cultivars. Boron seed priming proved beneficial under a specific range; however, deficiency (hydropriming) and excess (above 0.01% B) of B were detrimental for mungbean growth and productivity. The cultivar 'NM-2016' had significantly (p≤0.05) higher yield due to prominent increase in yield related traits with 0.01% B priming as compared to 'NM-2011'. In conclusion, B seed priming (0.01% B) seemed a feasible choice for improving mungbean growth, yield related traits and grain-B concentration of mungbean on Yermosols.
巴基斯坦耶尔莫索尔地区缺硼是限制绿豆生产力和谷物生物强化的主要因素之一。然而，硼（B）种子引发等农业策略有可能提高绿豆的产量和谷物的生物强化。此外，B 的缺乏到毒性范围非常狭窄；因此，在田间评估之前，必须优化其剂量。计划进行温室实验，以调查 B 种子引发对两种绿豆品种（即 NM-2011 和 NM-2016）的生长和品质的影响。使用了四种不同的 B 水平作为种子引发，即 0.01%、0.05%、0.1%和 1.0%B（硼砂 Na2B4O7.10H2O，11.5%B）进行了测试，而水引发被视为对照。用 0.01%B 进行种子引发显著（p≤0.05）降低了发芽开始时间和达到 50%出苗时间，而提高了平均出苗时间、出苗指数、最终出苗率、叶片数、根、茎和总重量的干重和鲜重、根长、株高、叶绿素含量、豆荚数和 100 粒重、每株种子数、每株植物的籽粒产量、茎和籽粒中的 B 浓度、籽粒蛋白质、碳水化合物和纤维在两个品种中。硼种子引发在特定范围内是有益的；然而，B 的缺乏（水引发）和过量（高于 0.01%B）对绿豆的生长和生产力都是有害的。与 NM-2011 相比，'NM-2016' 因 0.01%B 引发对产量相关性状的显著增加而具有显著（p≤0.05）更高的产量。总之，B 种子引发（0.01%B）似乎是改善耶尔莫索尔地区绿豆生长、产量相关性状和籽粒-B 浓度的可行选择。
