Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA.
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA; Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611.
Biochim Biophys Acta Biomembr. 2022 Jul 1;1864(7):183920. doi: 10.1016/j.bbamem.2022.183920. Epub 2022 Mar 28.
Viruses rely on hosts for their replication: thus, a critical step in the infection process is identifying a suitable host cell. Bacterial viruses, known as bacteriophages or phages, often use receptor binding proteins to discriminate between susceptible and non-susceptible hosts. By being able to evade predation, bacteria with modified or deleted receptor-encoding genes often undergo positive selection during growth in the presence of phage. Depending on the specific receptor(s) a phage uses, this may subsequently affect the bacteria's ability to form biofilms, its resistance to antibiotics, pathogenicity, or its phenotype in various environments. In this study, we characterize the interactions between two T4-like phages, Sf22 and KRT47, and their host receptor S. flexneri outer membrane protein C (OmpC). Results indicate that these phages use a variety of surface features on the protein, and that complete resistance most frequently occurs when hosts delete the ompC gene in full, encode premature stop codons to prevent OmpC synthesis, or eliminate specific regions encoding exterior loops.
因此，感染过程中的一个关键步骤是识别合适的宿主细胞。细菌病毒，称为噬菌体或噬菌体，通常使用受体结合蛋白来区分易感和不易感宿主。通过能够逃避捕食，具有修饰或缺失受体编码基因的细菌在噬菌体存在下生长时经常经历正选择。根据噬菌体使用的特定受体，这可能随后影响细菌形成生物膜的能力、对抗生素的耐药性、致病性或其在各种环境中的表型。在这项研究中，我们描述了两种 T4 样噬菌体 Sf22 和 KRT47 与其宿主受体 S. flexneri 外膜蛋白 C (OmpC) 之间的相互作用。结果表明，这些噬菌体使用蛋白质表面的多种特征，并且当宿主完全删除 ompC 基因、编码提前终止密码子以阻止 OmpC 合成或消除编码外环的特定区域时，最常发生完全抗性。
