Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan.
Department of United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
J Insect Physiol. 2022 May-Jun;139:104386. doi: 10.1016/j.jinsphys.2022.104386. Epub 2022 Mar 28.
There are various reports on embryogenesis in silkworm, Bombyx mori, a model organism for lepidopteran insects. New tissue observation methods have been developed with the development of biological science. Applying these methods to silkworm eggs makes it possible to capture morphological and histological features that have not been observed until now. Tissue transparency technology is a method of observation that has advanced remarkably recently. This study emphasized the CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) method. The tissue clearing reagent used in CUBIC is water-soluble, containing urea and amino alcohol, which easily and effectively clears animal tissue by immersing the sample in the reagent. This study showed that silkworm eggs are made transparent using the modified CUBIC method at the fertilization stage. Furthermore, we observed the behavior of egg nucleus, polar body nuclei, and sperm nuclei at the fertilization stage.
有关鳞翅目昆虫模式生物家蚕的胚胎发生，已有多种报告。随着生物科学的发展，新的组织观察方法也得到了发展。将这些方法应用于家蚕卵，使得能够捕捉到迄今为止尚未观察到的形态和组织学特征。组织透明技术是一种最近显著发展的观察方法。本研究强调了 CUBIC（透明、无阻碍的脑/体成像鸡尾酒和计算分析）方法。CUBIC 中使用的组织透明试剂是水溶性的，含有尿素和氨基醇，通过将样品浸入试剂中，很容易且有效地清除动物组织。本研究表明，在家蚕卵受精阶段，使用改良的 CUBIC 方法可使卵透明。此外，我们还观察了受精阶段卵核、极体核和精核的行为。
