Tao H, Liu H J, Cheng Y Q, Sima Y H, Yin W M, Xu S Q
Department of Applied Biology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, 215123, China.
J Comp Physiol B. 2015 Jan;185(1):47-55. doi: 10.1007/s00360-014-0864-6. Epub 2014 Sep 21.
Parental transcript legacy plays an important role in fertilization and development of the early embryo. Parental environmental exposure affects the fertilization of eggs, but the underlying biochemical mechanism is largely unresolved. In this study, the parental environmental effects on fertilization of eggs were explored in the silkworm Bombyx mori (B. mori), an ideal lepidopteran animal model. The results showed that the rate of fertilization decreased after the parents were exposed to a poor environment at 32 °C with continuous illumination for 72 h on days 6-9 of the pupal stage, which is a key period for germ cell maturation. This was likely attributable to lower energy charge values, obstructed nicotinamide adenine dinucleotide (NAD(+)) regeneration and inactive tricarboxylic acid cycle (TCA), leading to accumulation of large amounts of pyruvic acid and lactic acid. This effect was related to energy metabolism via glycolysis; in particular disruption of pyruvate metabolism. In conclusion, this study showed parental exposure to an abnormal environment during germ cell maturation affected glycolysis and the subsequent fertilization of eggs via the parental transcript legacy in B. mori.
亲本转录本遗产在早期胚胎的受精和发育中起着重要作用。亲本的环境暴露会影响卵子的受精，但潜在的生化机制在很大程度上尚未得到解决。在本研究中，以理想的鳞翅目动物模型家蚕为对象，探讨了亲本环境对卵子受精的影响。结果表明，在蛹期第6至9天，亲本在32°C、持续光照72小时的不良环境中暴露后，受精率下降，而这一时期是生殖细胞成熟的关键时期。这可能归因于较低的能荷值、烟酰胺腺嘌呤二核苷酸（NAD(+)）再生受阻以及三羧酸循环（TCA）不活跃，导致大量丙酮酸和乳酸积累。这种影响与通过糖酵解的能量代谢有关，尤其是丙酮酸代谢的紊乱。总之，本研究表明，在生殖细胞成熟过程中亲本暴露于异常环境会通过家蚕的亲本转录本遗产影响糖酵解及随后的卵子受精。
