Xinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Xinjiang key laboratory of new drug study and creation for herbivorous animal (XJ-KLNDSCHA), College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830000, PR China.
Poult Sci. 2022 May;101(5):101791. doi: 10.1016/j.psj.2022.101791. Epub 2022 Feb 18.
Poly (lactic-co-glycolic acid) (PLGA) nanoparticle used as vaccine adjuvants have been widely investigated due to their safety, antigen slow-release ability, and good adjuvants activity. In this study, immunopotentiator Alhagi honey polysaccharide encapsulated PLGA nanoparticles (AHPP) and assembled pickering emulsion with AHPP as shell and squalene as core (PPAS) were prepared. Characterization of AHPP and PPAS were investigated. H9N2 absorbed nanoparticles formulations were immunized to chicken, then the magnitude and kinetics of antibody and cellular immune responses were assessed. Our results showed that PPAS had rough strawberry-like surfaces, a large number of antigens could be absorbed on their surfaces through simple mixing. Adjuvant activity of PPAS showed that, PPAS/H9N2 can induce long-lasting and high HI titers, high thymus, spleen, and bursa of fabricius organ index. Moreover, chicken immunized with PPAS/H9N2 showed a mixed high differentiation of CD4 and CD8a T cell, and strong Th1 and Th2-type cytokines mRNA expression. Thus, these findings demonstrated that PPAS could induce a strong and long-term cellular and humoral immune response, and has the potential to serve as an effective vaccine delivery adjuvant system for H9N2 antigen.
聚（丙交酯-共-乙交酯）（PLGA）纳米粒子作为疫苗佐剂因其安全性、抗原缓慢释放能力和良好的佐剂活性而得到广泛研究。在这项研究中，我们制备了免疫增强剂沙棘蜜多糖包封的 PLGA 纳米粒子（AHPP）和 AHPP 作为壳层、角鲨烯作为核的 Pickering 乳液（PPAS）。对 AHPP 和 PPAS 进行了表征。将 H9N2 吸附纳米粒子制剂免疫鸡，然后评估抗体和细胞免疫应答的幅度和动力学。结果表明，PPAS 具有粗糙的草莓状表面，通过简单混合可以将大量抗原吸附在其表面上。PPAS 的佐剂活性表明，PPAS/H9N2 可以诱导持久和高 HI 滴度、高胸腺、脾脏和法氏囊器官指数。此外，用 PPAS/H9N2 免疫的鸡表现出 CD4 和 CD8a T 细胞的混合高分化，以及强烈的 Th1 和 Th2 型细胞因子 mRNA 表达。因此，这些发现表明 PPAS 可以诱导强烈和长期的细胞和体液免疫应答，并有可能成为 H9N2 抗原的有效疫苗递送佐剂系统。
