Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Neurochem Res. 2022 Jul;47(7):1991-2001. doi: 10.1007/s11064-022-03584-2. Epub 2022 Apr 1.
The field of tissue engineering exploits living cells in a variety of ways to restore, maintain, or enhance tissues and organs. Between stem cells, human induced pluripotent stem cells (hiPSCs), are very important due to their wide abilities. Growth factors can support proliferation, differentiation, and migration of hiPSCs. Platelet-rich plasma (PRP) could be used as the source of growth factors for hiPSCs. In the present study, proliferation and neural differentiation of hiPSCs on surface-modified nanofibrous Poly-L-lactic acid (PLLA) coated with platelet-rich plasma was investigated. The results of in vitro analysis showed that on the surface, which was modified nanofibrous scaffolds coated with platelet-rich plasma, significantly enhanced hiPSCs proliferation and neural differentiation were observed. Whereas the MTT ([3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide]) results showed biocompatibility of surface-modified nanofibrous scaffolds coated with platelet-rich plasma and the usage of these modified nanoscaffolds in neural tissue engineering in vivo is promising for the future.
组织工程领域利用各种方法利用活细胞来恢复、维持或增强组织和器官。在干细胞中，人类诱导多能干细胞（hiPSCs）因其广泛的能力而非常重要。生长因子可以支持 hiPSCs 的增殖、分化和迁移。富血小板血浆（PRP）可作为 hiPSCs 的生长因子来源。在本研究中，研究了表面修饰的富含血小板的血浆涂覆的纳米纤维聚 L-乳酸（PLLA）上 hiPSCs 的增殖和神经分化。体外分析结果表明，在表面修饰的富含血小板的血浆涂覆的纳米纤维支架上，观察到 hiPSCs 的增殖和神经分化显著增强。而 MTT（[3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐]）结果表明，表面修饰的富含血小板的血浆涂覆的纳米纤维支架具有生物相容性，并且这些修饰的纳米支架在体内神经组织工程中的应用具有广阔的前景。
