Hunan Provincial Key Laboratory of Micro and Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, People's Republic of China.
Department of Pharmacy, The Third Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Anal Sci. 2022 Mar;38(3):497-504. doi: 10.2116/analsci.20P455. Epub 2022 Apr 1.
Since microRNA-92a (miR-92a) and microRNA-21 (miR-21) are crucial biomarkers for colorectal cancer (CRC), monitoring miR-92a and miR-21 in serum is very significant for the early diagnosis of CRC. In this work, we developed a simple and sensitive fluorescent biosensor for the detection of miR-92a and miR-21 based on the quenching ability of Prussian blue nanoparticles (PBNPs) to fluorophores. Carboxyl fluorescein (FAM)-modified ssDNA (P-92a) and Cyanine 5 (Cy5)-modified ssDNA (P-21) were completely complementary to miR-92a and miR-21 separately. They were adsorbed on PBNPs surface by the binding of PO in DNA and Fe in PBNPs to fabricate the P-92a + P-21@PBNPs sensing system. The fluorescence responses from P-92a + P-21@PBNPs show good selection to miR-92a and a great linear process with the miR-92a concentration ranging from 1 to 30 nM (ΔF = 10.978 c + 71.457). Meanwhile, the fluorescence responses from P-92a + P-21@PBNPs is linearly relative to miR-21 from 3 to 30 nM; the linear equation is ΔF = 5.7560 c + 48.729. Furthermore, the detections of miR-92a and miR-21 added in serum samples were achieved. In summary, this method is sensitive, highly specific, time-saving, cost-effective and applicable for the detection of miR-92a and miR-21. Therefore, this present sensor was expected to be used in clinical applications, which lays a potential foundation for an early diagnosis of cancer.
由于 microRNA-92a（miR-92a）和 microRNA-21（miR-21）是结直肠癌（CRC）的重要生物标志物，因此监测血清中的 miR-92a 和 miR-21 对 CRC 的早期诊断非常重要。在这项工作中，我们开发了一种基于普鲁士蓝纳米粒子（PBNPs）对荧光团的淬灭能力的简单灵敏的荧光生物传感器，用于检测 miR-92a 和 miR-21。羧基荧光素（FAM）修饰的 ssDNA（P-92a）和 Cy5 修饰的 ssDNA（P-21）分别与 miR-92a 和 miR-21 完全互补。它们通过 DNA 中的 PO 和 PBNPs 中的 Fe 之间的结合吸附在 PBNPs 表面，以制备 P-92a+P-21@PBNPs 传感系统。来自 P-92a+P-21@PBNPs 的荧光响应对 miR-92a 具有良好的选择性，并且与 miR-92a 浓度在 1 至 30 nM 范围内呈很好的线性关系（ΔF=10.978 c+71.457）。同时，P-92a+P-21@PBNPs 的荧光响应与 3 至 30 nM 范围内的 miR-21 呈线性关系；线性方程为ΔF=5.7560 c+48.729。此外，还实现了血清样品中添加的 miR-92a 和 miR-21 的检测。总之，该方法具有灵敏度高、特异性高、耗时短、成本效益高、适用于 miR-92a 和 miR-21 的检测等优点。因此，这种传感器有望用于临床应用，为癌症的早期诊断奠定了潜在基础。
