The Whitney Laboratory for Marine Bioscience, St. Augustine, FL, USA.
Sorbonne Université, CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), Villefranche-sur-mer, France.
Methods Mol Biol. 2022;2450:95-119. doi: 10.1007/978-1-0716-2172-1_5.
Ctenophores, also known as comb jellies, are a clade of fragile holopelagic, carnivorous marine invertebrates, that represent one of the most ancient extant groups of multicellular animals. Ctenophores show a remarkable ability to regenerate in the adult form, being capable of replacing all body parts (i.e., whole-body regeneration) after loss/amputation. With many favorable experimental features (optical clarity, stereotyped cell lineage, multiple cell types), a full genome sequence available and their early branching phylogenetic position, ctenophores are well placed to provide information about the evolution of regenerative ability throughout the Metazoa. Here, we provide a collection of detailed protocols for use of the lobate ctenophore Mnemiopsis leidyi to study whole-body regeneration, including specimen collection, husbandry, surgical manipulation, and imaging techniques.
栉水母，又称栉水母，是一种脆弱的全海洋浮游、肉食海洋无脊椎动物，代表了最古老的现存多细胞动物群之一。栉水母在成体形式中具有显著的再生能力，在失去/截肢后能够替换所有身体部位（即全身再生）。栉水母具有许多有利的实验特征（光学透明度、定型的细胞谱系、多种细胞类型），拥有完整的基因组序列和早期分支的系统发育位置，非常适合提供有关整个后生动物再生能力进化的信息。在这里，我们提供了一套详细的实验方案，使用有齿栉水母 Mnemiopsis leidyi 来研究全身再生，包括标本收集、饲养、手术操作和成像技术。
