Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
University of Basel, Basel, Switzerland.
Methods Mol Biol. 2022;2450:619-633. doi: 10.1007/978-1-0716-2172-1_33.
Cells of the freshwater cnidarian Hydra possess an exceptional regeneration ability. In small groups of these cells, organizer centers emerge spontaneously and instruct the patterning of the surrounding population into a new animal. This property makes them an excellent model system to study the general rules of self-organization. A small tissue fragment or a clump of randomly aggregated cells can form a hollow spheroid that is able to establish a body axis de novo. Interestingly, mechanical oscillations (inflation/deflation cycles of the spheroid) driven by osmosis accompany the successful establishment of axial polarity. Here we describe different approaches for generating Hydra tissue spheroids, along with imaging and image analysis techniques to investigate their mechanical behavior.
淡水刺胞动物水螅的细胞具有非凡的再生能力。在这些细胞的小群体中，组织者中心自发出现，并指导周围群体的模式形成一个新的动物。这种特性使它们成为研究自组织一般规律的优秀模型系统。一小片组织或一团随机聚集的细胞可以形成一个能够从头建立身体轴的中空球体。有趣的是，由渗透作用驱动的机械振荡（球体的膨胀/收缩循环）伴随着轴向极性的成功建立。在这里，我们描述了生成水螅组织球体的不同方法，以及用于研究其机械行为的成像和图像分析技术。
