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lncRNA和mRNA表达谱的综合分析表明半月板存在与年龄相关的变化。

Integrated Analysis of lncRNA and mRNA Expression Profiles Indicates Age-Related Changes in Meniscus.

作者信息

Ai Li-Ya, Du Ming-Ze, Chen You-Rong, Xia Peng-Yan, Zhang Ji-Ying, Jiang Dong

机构信息

Department of Sports Medicine, Peking University Third Hospital, Beijing, China.

Institute of Sports Medicine of Peking University, Beijing, China.

出版信息

Front Cell Dev Biol. 2022 Mar 10;10:844555. doi: 10.3389/fcell.2022.844555. eCollection 2022.

DOI:10.3389/fcell.2022.844555
PMID:35359458
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8960627/
Abstract

Little has been known about the role of long non-coding RNA (lncRNA) involves in change of aged meniscus. Microarray analyses were performed to identify lncRNAs and mRNAs expression profiles of meniscus in young and aging adults and apple bioinformatics methods to analyse their potential roles. The differentially expressed (DE) lncRNAs and mRNAs were confirmed by qRT-PCR. A total of 1608 DE lncRNAs and 1809 DE mRNAs were identified. Functional and pathway enrichment analyses of all DE mRNAs showed that DE mRNAs were mainly involved in the TGF-beta, Wnt, Hippo, PI3K-Akt signaling pathway. The expressions of TNFRSF11B and BMP2 were significantly upregulated in aging group. LASSO logistic regression analysis of the DE lncRNAs revealed four lncRNAs (AC124312.5, HCG11, POC1B-AS1, and AP001011.1) that were associated with meniscus degradation. CNC analysis demonstrated that AP001011 inhibited the expression of TNFRSF11B and AC1243125 upregulated the expression of TNFRSF11B. CeRNA analysis suggested that POC1B-AS1 regulates the expression of BMP2 by sponging miR 130a-3p, miR136-5p, miR 18a-3p, and miR 608. Furthermore, subcellular localization and mA modification sites prediction analysis of these four lncRNAs was performed. These data lay a foundation for extensive studies on the role of lncRNAs in change of aged meniscus.

摘要

关于长链非编码RNA（lncRNA）在老年半月板变化中的作用，人们所知甚少。进行了微阵列分析，以确定年轻和老年成年人半月板的lncRNA和mRNA表达谱，并采用生物信息学方法分析其潜在作用。通过qRT-PCR对差异表达（DE）的lncRNA和mRNA进行了验证。共鉴定出1608个DE lncRNA和1809个DE mRNA。对所有DE mRNA的功能和通路富集分析表明，DE mRNA主要参与TGF-β、Wnt、Hippo、PI3K-Akt信号通路。衰老组中TNFRSF11B和BMP2的表达显著上调。对DE lncRNA进行LASSO逻辑回归分析，发现四个lncRNA（AC124312.5、HCG11、POC1B-AS1和AP001011.1）与半月板退变有关。CNC分析表明，AP001011抑制TNFRSF11B的表达，而AC1243125上调TNFRSF11B的表达。CeRNA分析表明，POC1B-AS1通过吸附miR 130a-3p、miR136-5p、miR 18a-3p和miR 608来调节BMP2的表达。此外，还对这四个lncRNA进行了亚细胞定位和m⁶A修饰位点预测分析。这些数据为深入研究lncRNA在老年半月板变化中的作用奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/b205a7041097/fcell-10-844555-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/e6b97d5e3562/fcell-10-844555-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/80bedff3f26c/fcell-10-844555-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/b969fd4e9c85/fcell-10-844555-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/9befd2485109/fcell-10-844555-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/b205a7041097/fcell-10-844555-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/e6b97d5e3562/fcell-10-844555-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/df5f16318d3d/fcell-10-844555-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/0fe00d2a7470/fcell-10-844555-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/dbcb6480c7cd/fcell-10-844555-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/3563a11e325f/fcell-10-844555-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/80bedff3f26c/fcell-10-844555-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/b969fd4e9c85/fcell-10-844555-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/9befd2485109/fcell-10-844555-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20c3/8960627/b205a7041097/fcell-10-844555-g009.jpg

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