Lin Yan, Wu De, Che Lianqiang, Fang Zhengfeng, Xu Shengyu, Feng Bin, Zhuo Yong, Li Jian, Wu Caimei, Zhang Junjie, Li Lujie
Key Laboratory of Animal Disease-Resistance Nutrition and Feed Science, Ministry of Agriculture, Sichuan Agricultural University, Chengdu, China.
Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Chengdu, China.
Front Vet Sci. 2022 Mar 11;9:850685. doi: 10.3389/fvets.2022.850685. eCollection 2022.
Testicular development is imperative to spermatogenesis, and pre-puberty is the key period for testis development. This study, therefore, investigated the effects of fibre supplementation on testis development and its possible mechanism in a growing boar model. Thirty Yorkshire boars were randomly divided into a control group (Control) and a fibre group (Fibre) from day 0 to 90 after weaning, with three pigs per pen and five pens per treatment. Blood and testes were collected for analysis. Dietary fibre supplementation had no significant effect on growth performance, testicular volume, or libido but increased the semen production of boars. Boars fed with fibre had lower serum cholesterol (CHO) and low-density lipoprotein (LDL) levels compared to those on the Control diet; however, testicular CHO, triglyceride (TG), and LDL concentration in the Fibre group were significantly higher than the Control group ( < 0.01). Testicular histological analysis showed that seminiferous tubules and testicular germ cells of 120-day-old boars were densely arranged in the Fibre group, and the number of Leydig cells was significantly higher than that of the Control group ( < 0.001). Furthermore, the diet supplemented with fibre significantly decreased leptin, leptin receptor (Leptor), and luteinising hormone (LH) concentrations in boar serum ( < 0.05), whereas follicle-stimulating hormone (FSH) and testosterone concentrations were significantly increased ( < 0.05). Meanwhile, the expression of , and genes related to proliferation and differentiation, and hormone-related genes and , were significantly up-regulated ( < 0.05). expression was up-regulated, whereas expression was down-regulated. In conclusion, increased fibre intake during the pre-puberty period in growing boar is crucial for Leydig cell proliferation, up-regulating the expression of genes related to hormone synthesis and thereby promoting the secretion of testosterone and semen production.
睾丸发育对精子发生至关重要，而青春期前是睾丸发育的关键时期。因此，本研究在生长中的公猪模型中，研究了补充纤维对睾丸发育的影响及其可能的机制。从断奶后第0天到90天，将30头约克夏公猪随机分为对照组（Control）和纤维组（Fibre），每栏3头猪，每个处理5栏。采集血液和睾丸进行分析。补充膳食纤维对公猪的生长性能、睾丸体积或性欲没有显著影响，但增加了公猪的精液产量。与对照组相比，饲喂纤维的公猪血清胆固醇（CHO）和低密度脂蛋白（LDL）水平较低；然而，纤维组睾丸中的CHO、甘油三酯（TG）和LDL浓度显著高于对照组（P<0.01）。睾丸组织学分析表明，纤维组120日龄公猪的生精小管和睾丸生殖细胞排列密集，睾丸间质细胞数量显著高于对照组（P<0.001）。此外，补充纤维的日粮显著降低了公猪血清中的瘦素、瘦素受体（Leptor）和促黄体生成素（LH）浓度（P<0.05），而促卵泡生成素（FSH）和睾酮浓度显著升高（P<0.05）。同时，与增殖和分化相关的基因以及与激素相关的基因的表达显著上调（P<0.05）。基因表达上调，而基因表达下调。总之，在生长中的公猪青春期前增加纤维摄入量对于睾丸间质细胞增殖、上调与激素合成相关的基因表达从而促进睾酮分泌和精液产生至关重要。
