Xu Yingyin, Zhang Zhiyuan, Feng Huiyu, Tang Jie, Peng Weihong, Chen Ying, Zhou Jie, Wang Yong
Sichuan Institute of Edible Fungi, Chengdu, China.
National-Local Joint Engineering Laboratory of Breeding and Cultivation of Edible and Medicinal Fungi, Chengdu, China.
Front Microbiol. 2022 Mar 10;13:865396. doi: 10.3389/fmicb.2022.865396. eCollection 2022.
, as an edible fungus, has multiple health benefits. However, the effects of on intestinal health are rarely explored. Hence, our study aims to elaborate on the influences of polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice. In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP). After 14-day administration, the jejunum and serum samples were collected for detection. The results showed that SSPs exert no effects on the growth performance of mice regardless of doses. Meanwhile, SSPs administration reduced the serum pro-inflammatory cytokines and elevated the anti-inflammatory cytokines. Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA. Mechanistically, the administration of SSPs enhanced the protein abundances of p-Nrf2, Keap1, and HO-1 in mice. The results of 16S rDNA demonstrated that the microbial community and composition were altered by SSPs administration. To summarize, SSPs benefit intestinal health in C57BL/6J mice a mechanism that involves elevating antioxidant and anti-inflammatory activities and regulating intestinal microbiota.
作为一种食用菌，具有多种健康益处。然而，其对肠道健康的影响鲜有研究。因此，我们的研究旨在阐述多糖（SSPs）对C57BL/6J小鼠抗氧化、抗炎及肠道微生物群的影响。在本研究中，18只雄性小鼠被随机分为三组：（1）对照组（CON）；（2）低剂量SSPs组（LSSP）；（3）高剂量SSPs组（HSSP）。给药14天后，收集空肠和血清样本进行检测。结果表明，无论剂量如何，SSPs对小鼠的生长性能均无影响。同时，SSPs给药降低了血清促炎细胞因子水平，提高了抗炎细胞因子水平。此外，SSPs给药提高了抗氧化能力，表现为总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）含量增加，丙二醛（MDA）含量降低。机制上，SSPs给药增强了小鼠中磷酸化核因子E2相关因子2（p-Nrf2）、 Kelch样环氧氯丙烷相关蛋白1（Keap1）和血红素氧合酶-1（HO-1）的蛋白丰度。16S核糖体DNA（16S rDNA）结果表明，SSPs给药改变了微生物群落和组成。综上所述，SSPs有益于C57BL/6J小鼠的肠道健康，其机制包括提高抗氧化和抗炎活性以及调节肠道微生物群。
