Institut Pasteur, Unité de Biologie des Bactéries Pathogènes à Gram-Positif, Paris, France.
Centre National de la Recherche Scientifique (CNRS) UMR6047, Paris, France.
Front Cell Infect Microbiol. 2022 Mar 11;12:794391. doi: 10.3389/fcimb.2022.794391. eCollection 2022.
subspecies () is an opportunistic pathogen causing invasive infections in the elderly often associated with colon neoplasia. The prevalence of in the stools of patients with normal colonoscopy (control) was compared with patients with colorectal adenomas (CRA) or with carcinomas (CRC) from stages I to IV. The presence of the s island encoding colibactin as well as other CRC-associated bacteria such as toxicogenic , , and was also investigated.
Fecal samples collected in France between 2011 and 2016 from patients with normal colonoscopy ( = 25), adenoma ( = 23), or colorectal cancer at different stages ( = 81) were tested by PCR for the presence of , , , , and the island. Relative quantification of , , and in stools was performed by qPCR.
prevalence was significantly increased in the CRC group. Our results also revealed i) a strong and significant increase of toxinogenic in patients with early-stage adenoma and of island at late-stage CRC and ii) increased levels of and in the stools of CRC patients. Furthermore, the simultaneous detection of these five bacterial markers was only found in CRC patients.
Our results indicate that the prevalence or relative levels of CRC-associated bacteria vary during CRC development. Among them, (+) was singled out as the sole pathobiont detected at the early adenoma stage.
亚种（）是一种机会性病原体，常引起老年人侵袭性感染，常与结肠肿瘤有关。比较了结肠镜检查正常的患者（对照组）粪便中的 与结直肠腺瘤（CRA）或 I 至 IV 期结肠癌（CRC）患者中的 。还研究了编码 colibactin 的 s 岛以及其他与 CRC 相关的细菌，如产毒 、 、 和 。
2011 年至 2016 年期间，在法国收集了结肠镜检查正常（=25）、腺瘤（=23）或不同阶段 CRC（=81）患者的粪便样本，通过 PCR 检测 、 、 、和 的存在。通过 qPCR 检测粪便中 、 和 的相对定量。
CRC 组的患病率显著增加。我们的结果还表明：i）早期腺瘤患者中产毒 显著增加，晚期 CRC 患者中 岛增加；ii）CRC 患者粪便中 和 的水平增加。此外，这些五种细菌标志物的同时检测仅在 CRC 患者中发现。
我们的研究结果表明，CRC 相关细菌的流行或相对水平在 CRC 发展过程中发生变化。其中，（+）被单独挑出，是在早期腺瘤阶段检测到的唯一条件致病菌。
