Xu Jun, Yang Min, Wang Dongyan, Zhang Shuilong, Yan Su, Zhu Yongliang, Chen Weichang
Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
Suzhou Precision Gene Biotechnology Co., Ltd., Suzhou, Jiangsu 215000, P.R. China.
Oncol Lett. 2020 Oct;20(4):106. doi: 10.3892/ol.2020.11967. Epub 2020 Aug 10.
() is reported to be associated with colorectal cancer (CRC). However, its association with colorectal adenoma (CRA) and its role in the initiation of colorectal tumors remain unknown. The present study aimed to clarify the relationship between and CRA and CRC by exploring the changes of abundance in an adenoma-carcinoma sequence in a new cohort and 4 public sequencing datasets. To investigate the alterations of abundance in the gut along the adenoma-carcinoma sequence, quantitative PCR (qPCR) was conducted to measure the relative abundance of in fecal samples from 277 subjects (128 patients with CRA, 66 patients with CRC and 83 healthy individuals, as controls) who underwent colonoscopy as outpatients. Then, the relative abundance of was analyzed in fecal samples from 596 subjects (185 healthy controls, 158 CRC, 253 CRA) in four public 16S rRNA sequencing datasets. The qPCR results demonstrated that the CRA group had an abundance of (P=0.2) similar to that of the healthy control group, while the CRC group had a significantly increased abundance (P=8.2×10). The level of effectively discriminated patients with CRC from healthy controls, while it poorly discriminated patients with CRA from healthy controls; with an area under the receiver operating characteristic curve of 0.867 for patients with CRC and 0.554 for patients with CRA. The same pattern of the alteration of abundance, which was low in healthy controls and patients with CRA but elevated in patients with CRC, was found in all four public sequencing datasets. These results suggested that was closely associated with, and may serve as a diagnostic marker for, CRC but not CRA. Moreover, it was indicated that may be an opportunistic pathogen of CRC, which may promote CRC development but serve a limited role in tumorigenesis.
据报道，（某物质）与结直肠癌（CRC）有关。然而，其与结直肠腺瘤（CRA）的关联以及在结直肠肿瘤发生中的作用仍不清楚。本研究旨在通过探索新队列和4个公共测序数据集中腺瘤-癌序列中（某物质）丰度的变化，阐明（某物质）与CRA和CRC之间的关系。为了研究沿腺瘤-癌序列肠道中（某物质）丰度的变化，对277名门诊接受结肠镜检查的受试者（128例CRA患者、66例CRC患者和83名健康个体作为对照）的粪便样本进行定量PCR（qPCR），以测量（某物质）的相对丰度。然后，在四个公共16S rRNA测序数据集中，对596名受试者（185名健康对照、158名CRC患者、253名CRA患者）的粪便样本中（某物质）的相对丰度进行分析。qPCR结果表明，CRA组（某物质）的丰度（P = 0.2）与健康对照组相似，而CRC组的丰度显著增加（P = 8.2×10）。（某物质）水平能有效区分CRC患者与健康对照，但区分CRA患者与健康对照的能力较差；CRC患者的受试者工作特征曲线下面积为0.867，CRA患者为0.554。在所有四个公共测序数据集中均发现了（某物质）丰度变化的相同模式，即健康对照和CRA患者中较低，而CRC患者中升高。这些结果表明，（某物质）与CRC密切相关，可能作为CRC的诊断标志物，但与CRA无关。此外，表明（某物质）可能是CRC的机会致病菌，其可能促进CRC发展，但在肿瘤发生中作用有限。
