Khairani Shafia, Fauziah Nisa, Wiraswati Hesti Lina, Panigoro Ramdan, Setyowati Endang Yuni, Berbudi Afiat
Doctoral Program, Medical Science, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia.
Veterinary Medicine Program, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia.
J Trop Med. 2022 Mar 22;2022:5721449. doi: 10.1155/2022/5721449. eCollection 2022.
Malaria remains a public health problem and a leading cause of death worldwide. Consequently, the discovery of novel agents, including substances from medicinal plants, is urgently needed. has long been used by the community in the treatment of the symptoms of malaria. In a previous study, was demonstrated to exhibit promising antiplasmodial activity against 3D7 and INDO strains. The aim of this study was to further investigate the antimalarial activity (curative and prophylactic) of piperine (a major isolated constituent of in ANKA-infected mice. Piperine 10, 20, and 40 mg/kg body weight (bw), artesunate 5 mg/kg bw, and DMSO were administered orally for four days to different groups of Swiss Webster mice. Then, mice were monitored for parasitaemia, body weight, rectal temperature, survival rate, and clinical parameters. Piperine 40 mg/kg bw in curative and prophylactic tests had the maximum parasitaemia chemosuppression of 79.21% and 58.8% ( < 0.05), respectively, with a significant effect on the survival rate compared with control animals. In the curative test, piperine 40 mg/kg bw reduced the mean clinical score compared with the control group. Additionally, piperine showed an ability to protect organs (lungs, liver, spleen, and kidneys) from some damage in a dose-dependent manner. This study can be used as a basis for further discovery of novel chemotherapeutic or chemoprophylactic compounds.
疟疾仍然是一个公共卫生问题，是全球主要的死亡原因。因此，迫切需要发现新型药物，包括来自药用植物的物质。 长期以来一直被社区用于治疗疟疾症状。在先前的一项研究中，已证明 对3D7和INDO菌株具有有前景的抗疟原虫活性。本研究的目的是进一步研究胡椒碱（ 的主要分离成分）在感染ANKA的小鼠中的抗疟活性（治疗性和预防性）。将10、20和40mg/kg体重（bw）的胡椒碱、5mg/kg bw的青蒿琥酯和二甲基亚砜口服给予不同组的瑞士韦伯斯特小鼠，持续四天。然后，监测小鼠的寄生虫血症、体重、直肠温度、存活率和临床参数。在治疗性和预防性试验中，40mg/kg bw的胡椒碱对寄生虫血症的最大化学抑制率分别为79.21%和58.8%（ ＜0.05），与对照动物相比，对存活率有显著影响。在治疗性试验中，40mg/kg bw的胡椒碱与对照组相比降低了平均临床评分。此外，胡椒碱显示出以剂量依赖的方式保护器官（肺、肝、脾和肾）免受一些损伤的能力。本研究可作为进一步发现新型化疗或化学预防化合物的基础。
