Zhang Wei, Zhou Xiaoying, Chen Feng, Zhu Hongli, Shi Rui, Sun Chengming, Chen Song, Hu Maolong, Zhang Jiefu, Wang Xiaodong
Key Laboratory of Jiangsu Province for Agrobiology, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2022 Mar 10;13:850330. doi: 10.3389/fpls.2022.850330. eCollection 2022.
Chloroplast development is crucial for photosynthesis and plant growth and many factors are involved in its regulation. The regulatory mechanism differs in different green tissues, and previous studies have focused on chloroplasts in leaves. In this study, a mutant with sepal-specific chlorophyll-deficiency was observed in and named as df74. Genetic analysis indicated that the phenotype was controlled by a single recessive nuclear gene. The gene was located on chromosome C08 by bulked-segregant analysis with whole-genome sequencing, which was designated as . To fine-map the , a F population was created from the cross of df74 and Zhongshuang11 (ZS11). Finally, the was fine-mapped in the region between the single-nucleotide polymorphism (SNP) markers M5 and M6, corresponding to a 228.72 kb interval of the "ZS11" genome. Eighteen genes were predicted in the target region, and it was speculated that was the most likely candidate gene based on the results of transcriptome analyses and sequence variation analyses. These results provide a foundation to explore the regulation of chloroplast development in sepals.
叶绿体发育对于光合作用和植物生长至关重要，且有许多因素参与其调控。调控机制在不同绿色组织中有所不同，先前的研究主要集中在叶片中的叶绿体。在本研究中，在[具体材料]中观察到一个具有萼片特异性叶绿素缺乏的突变体，并将其命名为df74。遗传分析表明该表型受单个隐性核基因控制。通过全基因组测序的混合分组分析法将该基因定位在C08染色体上，命名为[具体基因名]。为了精细定位[具体基因名]，利用df74与中双11（ZS11）杂交创建了一个F[具体世代]群体。最终，将[具体基因名]精细定位在单核苷酸多态性（SNP）标记M5和M6之间的区域，对应于“ZS11”基因组的228.72 kb区间。在目标区域预测到18个基因，基于转录组分析和序列变异分析结果推测[具体基因名]是最有可能的候选基因。这些结果为探索萼片中叶绿体发育的调控提供了基础。
