State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, China.
Soybean Research Institute, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
Sci Rep. 2016 Aug 10;6:31419. doi: 10.1038/srep31419.
Leaf colour regulation is important in photosynthesis and dry material production. Most of the reported chlorophyll-deficient loci are recessive. The dominant locus is rarely reported, although it may be more important than the recessive locus in the regulation of photosynthesis efficiency. During the present study, we mapped a chlorophyll-deficient dominant locus (CDE1) from the ethyl methanesulfonate-mutagenized Brassica napus line NJ7982. Using an F2 population derived from the chlorophyll-deficient mutant (cde1) and the canola variety 'zhongshuang11', a high-density linkage map was constructed, consisting of 19 linkage groups with 2,878 bins containing 13,347 SNP markers, with a total linkage map length of 1,968.6 cM. Next, the CDE1 locus was mapped in a 0.9-cM interval of chromosome C08 of B. napus, co-segregating with nine SNP markers. In the following fine-mapping of the gene using the inherited F2:3 populations of 620 individuals, the locus was identified in an interval with a length of 311 kb. A bioinformatics analysis revealed that the mapping interval contained 22 genes. These results produced a good foundation for continued research on the dominant locus involved in chlorophyll content regulation.
叶片颜色调控对光合作用和干物质生产很重要。已报道的大多数叶绿素缺失基因均为隐性。显性基因很少报道，尽管其在调控光合作用效率方面可能比隐性基因更为重要。本研究中，我们从乙磺酸乙酯诱变的甘蓝型油菜品系 NJ7982 中定位了一个叶绿素缺失显性基因（CDE1）。利用叶绿素缺失突变体（cde1）与油菜品种‘中双 11’衍生的 F2 群体，构建了一个包含 19 个连锁群、2878 个 bin、包含 13347 个 SNP 标记的高密度连锁图谱，总图谱长度为 1968.6cM。随后，将 CDE1 基因定位到甘蓝型油菜 C08 染色体的 0.9cM 区间内，与 9 个 SNP 标记共分离。在随后使用遗传的 F2:3 群体（620 个个体）对基因的精细定位中，将该基因定位于 311kb 的区间内。生物信息学分析显示，该定位区间包含 22 个基因。这些结果为进一步研究参与叶绿素含量调控的显性基因奠定了良好基础。
