Saqib Fatima, Al-Huqail Arwa Abdulkreem, Asma Memona, Chicea Liana, Hogea Mircea, Irimie Marius, Gavris Claudia
Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
Biology Department, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Dose Response. 2022 Mar 27;20(1):15593258221079592. doi: 10.1177/15593258221079592. eCollection 2022 Jan-Mar.
L. is a medicinally effective plant used in indigenous system of medicine for a variety of ailments. However, there is no comprehensive study explaining its effectiveness in gastrointestinal tract, respiratory, and cardiovascular system ailments. This study was designed to validate the pharmacological basis for the folkloric use of L. in diarrhea, asthma, and hypertension. extract was analyzed to detect the presence of bioactive compounds by HPLC. The isolated rabbit jejunum, trachea, and aorta were used for in vitro experiments using tissue bath assembly coupled with Power Lab data acquisition system to explore their relative effects. experiments were performed for anti-diarrheal activity. HPLC analysis revealed the presence of gallic acid, butylated hydroxytoluene, catechin, and quercetin. Concentration dependent activities were observed by relaxing K (low) induced contractions having spasmolytic effect with EC50 = .358 ± .052, bronchodilator (EC50 = 2.483 ± .05793), and vasorelaxant (EC50 = .383 ± .063), probably due to the ATP dependent potassium channel activation. It was confirmed through pre-exposure of glibenclamide (specific ATP-dependent K channel blocker) having similarities with cromakalim. Pm.Cr revealed its antidiarrheal via in vivo experiments on rats. This study indicates that has antidiarrheal, spasmolytic, bronchodilator, and vasorelaxant activities probably due to the ATP dependent K channel activation.
L. 是一种在传统医学体系中用于治疗多种疾病的药用植物。然而，目前尚无全面的研究来解释其在胃肠道、呼吸系统和心血管系统疾病中的有效性。本研究旨在验证L. 在腹泻、哮喘和高血压民间应用的药理学基础。通过高效液相色谱法（HPLC）分析提取物以检测生物活性化合物的存在。使用离体兔空肠、气管和主动脉，通过组织浴装置结合Power Lab数据采集系统进行体外实验，以探究其相关作用。进行实验以研究其抗腹泻活性。HPLC分析显示存在没食子酸、丁基化羟基甲苯、儿茶素和槲皮素。观察到浓度依赖性活性，其通过舒张钾（低）诱导的收缩发挥解痉作用，EC50 = 0.358 ± 0.052，具有支气管扩张作用（EC50 = 2.483 ± 0.05793）和血管舒张作用（EC50 = 0.383 ± 0.063），这可能是由于ATP依赖性钾通道激活所致。通过预先暴露与克罗卡林相似的格列本脲（特异性ATP依赖性钾通道阻滞剂）得以证实。Pm.Cr通过对大鼠的体内实验显示出其抗腹泻作用。本研究表明，L. 可能由于ATP依赖性钾通道激活而具有抗腹泻、解痉、支气管扩张和血管舒张活性。
