Janbaz Khalid H, Javed Sana, Saqib Fatima, Imran Imran, Zia-Ul-Haq Muhammad, De Feo Vincenzo
Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
Department of Pharmacognosy, University of Karachi, Karachi, Pakistan.
BMC Complement Altern Med. 2015 Jun 6;15:169. doi: 10.1186/s12906-015-0697-1.
Heliotropium strigosum is used in traditional medicine to manage gastrointestinal pain, respiratory distress and vascular disorders. The present study was undertaken to provide scientific evidences for these folkloric uses by in vitro experimental settings.
A crude methanol extract of the Heliotropium strigosum (Hs.Cr) was tested in vitro on isolated rabbit jejunum preparations to detect the possible presence of spasmolytic activity. Moreover, isolated rabbit tracheal and aorta preparations were used to ascertain the relaxant effects of the extract.
The Hs.Cr exhibited relaxant effects in rabbit jejunum in a concentration dependent manner (0.01-3.0 mg/ml). The Hs.Cr also relaxed K(+) (80 mM)-induced spastic contractions in rabbit jejunum and shifted the Ca(2+) concentration response curves towards right. The extract relaxed carbachol (1 μM)- as well as K(+) (80 mM)-induced contractions in rabbit trachea at concentrations ranging from 0.01 to 10 mg/ml. Moreover, Hs.Cr. also relaxed (0.01-3.0 mg/ml) the phenylephrine (1 μM)- and K(+) (80 mM)-induced contractions in isolated rabbit aorta.
The Hs.Cr was found to exhibit spasmolytic, bronchodilator and vasorelaxant activities on isolated rabbit jejunum, trachea and aorta preparations, likely mediated through Ca(2+) channel blockade. This finding may provide a scientific basis for the folkloric uses of the plant.
硬毛天芥菜在传统医学中用于治疗胃肠道疼痛、呼吸窘迫和血管疾病。本研究旨在通过体外实验为这些民间用途提供科学依据。
对硬毛天芥菜的粗甲醇提取物（Hs.Cr）在离体兔空肠制剂上进行体外测试，以检测是否可能存在解痉活性。此外，使用离体兔气管和主动脉制剂来确定该提取物的舒张作用。
Hs.Cr对兔空肠表现出浓度依赖性的舒张作用（0.01 - 3.0 mg/ml）。Hs.Cr还能缓解K⁺（80 mM）诱导的兔空肠痉挛性收缩，并使Ca²⁺浓度反应曲线右移。该提取物在0.01至10 mg/ml的浓度范围内可缓解卡巴胆碱（1 μM）以及K⁺（80 mM）诱导的兔气管收缩。此外，Hs.Cr（0.01 - 3.0 mg/ml）还能缓解苯肾上腺素（1 μM）和K⁺（80 mM）诱导的离体兔主动脉收缩。
发现Hs.Cr对离体兔空肠、气管和主动脉制剂具有解痉、支气管舒张和血管舒张活性，可能是通过Ca²⁺通道阻滞介导的。这一发现可能为该植物的民间用途提供科学依据。
