Yang Ni, Zhan Yang, Wan Jianhua, Li Yingmeng, Hu Xu, Liu Wenjun
Key Laboratory of Taste Correction (Taste Masking) and Sensory Evaluation of Traditional Chinese Medicine, Jiangzhong Pharmaceutical Co., Ltd., Nanchang, Jiangxi 330096, China.
Can J Infect Dis Med Microbiol. 2022 Mar 22;2022:6521793. doi: 10.1155/2022/6521793. eCollection 2022.
Antibiotic-associated diarrhea (AAD) is a common morbidity caused by antibiotic use and is characterized by the dysbiosis of the gut microbiota. Several clinical trials have shown that probiotics can prevent AAD. This study aimed at investigating the effects of Lacidophilin tablets (LB), yogurt (YG), and bifid triple viable capsules (BT) on the gut microbiota of mice with AAD. Mice with diarrhea were randomly allocated to treatment groups or the control group and were treated with either LB, YG, BT, or vehicle control. The body weight, diarrhea scores, cecum index, and cecal length were determined. Fecal samples of all mice were analyzed using 16S rRNA high-throughput sequencing. The results showed that LB, YG, and BT significantly decreased the diarrhea scores and inhibited increases in the cecum index and cecal length induced by AAD. In addition, they significantly changed the composition and richness of the gut microbiota. Specifically, they increased the abundance of the phylum Firmicutes and decreased the abundance of the phyla Bacteroidetes and the family Bacteroidaceae. Treatment with LB and YG also decreased the abundance of the phylum Proteobacteria and only LB could mediate the reduced levels of Lactobacillaceae in AAD mice. At the genus level, YG and BT treatment decreased the abundance of or . To our surprise, only LB treatment dramatically increased the abundance of and decreased that of potential pathogens, such as , and , to almost normal values. Our findings indicate that LB, YG, and BT ameliorated diarrhea by regulating the composition and structure of the gut microbiota and that LB plays an important role in regulating the gut microbiota.
抗生素相关性腹泻（AAD）是由抗生素使用引起的常见病症，其特征为肠道微生物群失调。多项临床试验表明，益生菌可预防AAD。本研究旨在探讨嗜酸乳杆菌片（LB）、酸奶（YG）和双歧三联活菌胶囊（BT）对AAD小鼠肠道微生物群的影响。将腹泻小鼠随机分为治疗组或对照组，分别用LB、YG、BT或赋形剂对照进行治疗。测定体重、腹泻评分、盲肠指数和盲肠长度。使用16S rRNA高通量测序分析所有小鼠的粪便样本。结果表明，LB、YG和BT显著降低了腹泻评分，并抑制了AAD诱导的盲肠指数和盲肠长度增加。此外，它们显著改变了肠道微生物群的组成和丰富度。具体而言，它们增加了厚壁菌门的丰度，降低了拟杆菌门和拟杆菌科的丰度。LB和YG治疗也降低了变形菌门的丰度，只有LB能介导AAD小鼠中乳酸菌科水平的降低。在属水平上，YG和BT治疗降低了 或 的丰度。令我们惊讶的是，只有LB治疗显著增加了 的丰度，并将潜在病原体如 、 和 的丰度降低到几乎正常水平。我们的研究结果表明，LB、YG和BT通过调节肠道微生物群的组成和结构改善腹泻，并且LB在调节肠道微生物群中起重要作用。
