Granato P A, Giachetti-Powers L M, Murfitt K S, King D
Diagn Microbiol Infect Dis. 1986 Nov;5(4):293-8. doi: 10.1016/0732-8893(86)90031-3.
The PathoDx Strep A kit, a 10-min acid extraction and latex agglutination test, was compared with routine culture for the direct detection of group A beta-hemolytic streptococci (BHS) in 414 pharyngeal specimens collected from children with pharyngitis. The results showed that the latex test compared favorably with culture for detecting group A BHS in pharyngeal specimens (sensitivity 96.7%, specificity 97.9%, and positive and negative predictive values of 97.2% and 97.4%, respectively). The comparable number of false-positive (five) and false-negative (six) latex tests along with review of patient histories suggest that these discrepant results were attributable to sampling error during specimen procurement rather than deficiencies in the latex kit. In addition, clear-cut, agglutination reactions were obtained in over 96% of positive latex tests regardless of the amount of group A BHS that was recovered by culture. The PathoDx Strep A test is a rapid, reliable noncultural alternative for the detection of group A BHS in pharyngeal specimens.
将PathoDx A群链球菌检测试剂盒（一种需10分钟的酸提取乳胶凝集试验）与常规培养法进行比较，以直接检测从咽炎患儿采集的414份咽拭子标本中的A群β溶血性链球菌（BHS）。结果显示，在检测咽拭子标本中的A群BHS方面，乳胶试验与培养法相比具有优势（敏感性96.7%，特异性97.9%，阳性和阴性预测值分别为97.2%和97.4%）。乳胶试验出现的假阳性（5例）和假阴性（6例）数量相当，结合患者病史回顾表明，这些差异结果归因于标本采集过程中的抽样误差，而非乳胶试剂盒存在缺陷。此外，无论培养法回收的A群BHS数量多少，超过96%的阳性乳胶试验都能获得清晰的凝集反应。PathoDx A群链球菌检测试验是一种快速、可靠的非培养方法，可用于检测咽拭子标本中的A群BHS。
