Gen-Probe A群链球菌直接检测法与培养法及快速链球菌抗原检测法在诊断链球菌性咽炎中的比较。
Comparison of the Gen-Probe Group A streptococcus Direct Test with culture and a rapid streptococcal antigen detection assay for diagnosis of streptococcal pharyngitis.
作者信息
Heiter B J, Bourbeau P P
机构信息
Department of Laboratory Medicine, Geisinger Medical Center, Danville, Pennsylvania 17822.
出版信息
J Clin Microbiol. 1993 Aug;31(8):2070-3. doi: 10.1128/jcm.31.8.2070-2073.1993.
Abstract
The Gen-Probe Group A Streptococcus Direct Test (GP-ST) is a new assay which utilizes a nucleic acid probe to detect group A streptococci directly from pharyngeal swabs. In this study, 1,103 specimens were cultured and tested by GP-ST. The sensitivities and specificities were as follows: culture, 98.8 and 100%; GP-ST, 92.4 and 99.6%. Of the 1,103 specimens, 808 were also tested with the TestPack Strep A assay. For the specimens tested by all three methods, the sensitivities and specificities were as follows: culture, 99.5 and 100%; TestPack Strep A assay, 76.3 and 99.7%; GP-ST, 93.5 and 99.7%. The GP-ST is a very user-friendly assay which has the potential to replace culture for the diagnosis of streptococcal pharyngitis.
摘要
基因探针A群链球菌直接检测试验（GP - ST）是一种新的检测方法，它利用核酸探针直接从咽拭子中检测A群链球菌。在本研究中，对1103份标本进行了培养并用GP - ST检测。敏感性和特异性如下：培养法，98.8%和100%；GP - ST，92.4%和99.6%。在1103份标本中，808份还采用了A群链球菌快速检测试剂盒进行检测。对于用所有三种方法检测的标本，敏感性和特异性如下：培养法，99.5%和100%；A群链球菌快速检测试剂盒，76.3%和99.7%；GP - ST，93.5%和99.7%。GP - ST是一种非常便于使用的检测方法，有潜力取代培养法用于链球菌性咽炎的诊断。
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