Kim M K, Palestine A G, Nussenblatt R B, Chan C C
Curr Eye Res. 1986 Nov;5(11):869-76. doi: 10.3109/02713688609029239.
Uveitis can be induced by systemic or intravitreal administration of endotoxin (lipopolysaccharide, LPS). In this study we correlated the expression of class II antigens (la in rat) of the Major Histocompatibility Complex (MHC), with this experimental model of uveitis. Ia antigen was detected by immunohistochemistry using the Avidin-Biotin-Peroxidase Complex (ABC) method and the monoclonal antibody OX6. Ia antigen was not expressed in normal eyes. However, Ia was expressed in the anterior uvea epithelial cells in all eyes with LPS induced uveitis. This study demonstrates that the ocular Ia expression is a localized process in the anterior uvea in response to systemic or intravitreal LPS. This response appears to be distinct from the action of LPS on macrophage Ia expression, where LPS has been shown to inhibit the induction of Ia antigen in macrophages by gamma interferon.
葡萄膜炎可由全身或玻璃体内注射内毒素（脂多糖，LPS）诱导产生。在本研究中，我们将主要组织相容性复合体（MHC）II类抗原（大鼠中的Ia）的表达与这种葡萄膜炎实验模型相关联。使用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）方法和单克隆抗体OX6通过免疫组织化学检测Ia抗原。正常眼中未表达Ia抗原。然而，在所有LPS诱导的葡萄膜炎眼中，Ia在前葡萄膜上皮细胞中表达。本研究表明，眼部Ia表达是前葡萄膜中对全身或玻璃体内LPS的一种局部反应过程。这种反应似乎与LPS对巨噬细胞Ia表达的作用不同，在巨噬细胞中，LPS已被证明可抑制γ干扰素对Ia抗原的诱导。
