Guex-Crosier Y, Wittwer A J, Roberge F G
National Institutes of Health, National Eye Institute, Bethesda, Maryland, USA.
Br J Ophthalmol. 1996 Jul;80(7):649-53. doi: 10.1136/bjo.80.7.649.
AIMS/BACKGROUND: The subcutaneous injection of bacterial endotoxin in Lewis rats produces an acute intraocular inflammation evolving over a 24 hour period. This endotoxin induced uveitis (EIU) is characterised by a biphasic protein exudation and a cellular infiltrate composed of macrophages and polymorphonuclear neutrophils (PMNs). This model was used to study the mechanism of cellular infiltration in ocular inflammation.
EIU was induced by a subcutaneous injection of lipopolysaccharide (LPS) (S typhimurium) at 350 micrograms/kg. The levels of cytokine induced neutrophil chemoattractant (CINC) were measured every 2 hours in the serum and in the aqueous humour by ELISA. The intraocular inflammation was quantified by protein measurement and leucocyte counting.
The kinetics of CINC production in the systemic circulation showed a rapid rise, peaking 2 hours after LPS injection, followed by a progressive decline over the next 8 hours. In the eye, the CINC levels increased above the serum levels 10 hours after EIU induction corresponding to the time of cellular infiltration. When leucocyte entry in the eye was inhibited by 56% and 64% with an antiadhesion molecule antibody, there was only a slight reduction in the aqueous humour CINC levels of 9% and 16%, respectively, indicating that CINC was produced by ocular tissue cells. The specific effect of CINC in the eye was confirmed when a direct intraocular injection of 250 ng of purified CINC was followed by significant PMN infiltration, in the absence of protein exudation.
The data indicate that the production of the CINC chemotactic factor by ocular tissue participates in the inflammatory reaction in EIU.
目的/背景：在Lewis大鼠皮下注射细菌内毒素可引发急性眼内炎症，该炎症在24小时内逐渐发展。这种内毒素诱导的葡萄膜炎（EIU）的特征是双相性蛋白质渗出以及由巨噬细胞和多形核中性粒细胞（PMN）组成的细胞浸润。此模型用于研究眼内炎症中细胞浸润的机制。
通过皮下注射350微克/千克的脂多糖（LPS）（鼠伤寒沙门氏菌）诱导EIU。每2小时通过ELISA法测定血清和房水中细胞因子诱导的中性粒细胞趋化因子（CINC）的水平。通过蛋白质测量和白细胞计数对眼内炎症进行定量。
全身循环中CINC产生的动力学显示其迅速升高，在LPS注射后2小时达到峰值，随后在接下来的8小时内逐渐下降。在眼部，EIU诱导10小时后CINC水平升高至高于血清水平，这与细胞浸润的时间相对应。当用抗粘附分子抗体使进入眼内的白细胞减少56%和64%时，房水中CINC水平仅分别轻微降低9%和16%，表明CINC是由眼组织细胞产生 的。当直接眼内注射250纳克纯化的CINC后出现显著的PMN浸润且无蛋白质渗出时，证实了CINC在眼中的特定作用。
数据表明眼组织产生的CINC趋化因子参与了EIU中的炎症反应。
